Koutsos Athanasios, Jackson Kim G, Lockyer Stacey, Carvalho-Wells Andrew, Minihane Anne M, Lovegrove Julie A
Hugh Sinclair Unit of Human Nutrition, Institute for Cardiovascular and Metabolic Research (ICMR), Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UK.
Hugh Sinclair Unit of Human Nutrition, Institute for Cardiovascular and Metabolic Research (ICMR), Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UK.
Cytokine. 2014 Apr;66(2):156-9. doi: 10.1016/j.cyto.2013.12.015. Epub 2014 Jan 29.
Apolipoprotein E (APOE) genotype is believed to play an important role in cardiovascular risk. APOE4 carriers have been associated with higher blood lipid levels and a more pro-inflammatory state compared with APOE3/E3 individuals. Although dietary fat composition has been considered to modulate the inflammatory state in humans, very little is known about how APOE genotype can impact on this response. In a follow-up to the main SATgenε study, we aimed to explore the effects of APOE genotype, as well as, dietary fat manipulation on ex vivo cytokine production. Blood samples were collected from a subset of SATgenε participants (n=52/88), prospectively recruited according to APOE genotype (n=26 E3/E3 and n=26 E3/E4) after low-fat (LF), high saturated fat (HSF) and HSF with 3.45g docosahexaenoic acid (DHA) dietary periods (each diet eight weeks in duration assigned in the same order) for the measurement of ex vivo cytokine production using whole blood culture (WBC). Concentrations of IL-1beta, IL-6, IL-8, IL-10 and TNF-alpha were measured in WBC supernatant samples after stimulation for 24h with either 0.05 or 1μg/ml of bacterial lipopolysaccharide (LPS). Cytokine levels were not influenced by genotype, whereas, dietary fat manipulation had a significant impact on TNF-α and IL-10 production; TNF-α concentration was higher after consumption of the HSF diet compared with baseline and the LF diet (P<0.05), whereas, IL-10 concentration was higher after the LF diet compared with baseline (P<0.05). In conclusion, our study has revealed the amount and type of dietary fat can significantly modulate the production of TNF-α and IL-10 by ex vivo LPS-stimulated WBC samples obtained from normolipidaemic subjects.
载脂蛋白E（APOE）基因型被认为在心血管风险中起重要作用。与APOE3/E3个体相比，APOE4携带者的血脂水平更高，且处于更具促炎状态。尽管饮食脂肪组成被认为可调节人类的炎症状态，但关于APOE基因型如何影响这种反应却知之甚少。在SATgenε主要研究的后续研究中，我们旨在探讨APOE基因型以及饮食脂肪操控对体外细胞因子产生的影响。从SATgenε参与者的一个亚组（n = 52/88）中采集血样，这些参与者根据APOE基因型（n = 26 E3/E3和n = 26 E3/E4）进行前瞻性招募，在经历低脂（LF）、高饱和脂肪（HSF）以及添加3.45克二十二碳六烯酸（DHA）的HSF饮食期（每种饮食持续八周，按相同顺序安排）后，使用全血培养（WBC）来测量体外细胞因子产生。在用0.05或1μg/ml细菌脂多糖（LPS）刺激24小时后，测量WBC上清液样本中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、白细胞介素-10（IL-10）和肿瘤坏死因子-α（TNF-α）的浓度。细胞因子水平不受基因型影响，然而，饮食脂肪操控对TNF-α和IL-10的产生有显著影响；与基线和LF饮食相比，食用HSF饮食后TNF-α浓度更高（P<0.05），而与基线相比，LF饮食后IL-10浓度更高（P<0.05）。总之，我们的研究表明，饮食脂肪的量和类型可显著调节从血脂正常受试者获得的体外LPS刺激的WBC样本中TNF-α和IL-10的产生。
