采用直流电刺激进行周围神经的体外电泳和体内电生理学研究。
In vitro electrophoresis and in vivo electrophysiology of peripheral nerve using DC field stimulation.
机构信息
Surgery Department, Duke University Medical Center, Durham, NC 27710, United States; Research Service of the Veterans Affairs Medical Center, Durham, NC 27705, United States.
Surgery Department, Duke University Medical Center, Durham, NC 27710, United States.
出版信息
J Neurosci Methods. 2014 Mar 30;225:90-6. doi: 10.1016/j.jneumeth.2014.01.018. Epub 2014 Jan 29.
BACKGROUND
Given the movement of molecules within tissue that occurs naturally by endogenous electric fields, we examined the possibility of using a low-voltage DC field to move charged substances in rodent peripheral nerve in vitro.
NEW METHOD
Labeled sugar- and protein-based markers were applied to a rodent peroneal nerve and then a 5-10 V/cm field was used to move the molecules within the extra- and intraneural compartments. Physiological and anatomical nerve properties were also assessed using the same stimulation in vivo.
RESULTS
We demonstrate in vitro that charged and labeled compounds are capable of moving in a DC field along a nerve, and that the same field applied in vivo changes the excitability of the nerve, but without damage.
CONCLUSIONS
The results suggest that low-voltage electrophoresis could be used to move charged molecules, perhaps therapeutically, safely along peripheral nerves.
背景
鉴于组织内分子在天然存在的内源性电场作用下的运动，我们研究了利用低电压直流电在体外移动啮齿动物周围神经中带电荷物质的可能性。
新方法
将标记的糖基和蛋白基标记物应用于啮齿动物腓神经，然后使用 5-10 V/cm 的电场来移动神经外和神经内隔室中的分子。还使用相同的刺激在体内评估了神经的生理和解剖学特性。
结果
我们在体外证明了带电荷和标记的化合物能够在直流电场中沿着神经移动，并且体内施加的相同电场会改变神经的兴奋性，但不会造成损伤。
结论
结果表明，低电压电泳可用于安全地沿周围神经移动带电荷的分子，也许可用于治疗。
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