Duplancic Bozidar, Stambolija Vasilije, Holjevac Jadranka, Zemba Mladen, Balenovic Igor, Drmic Domagoj, Suran Jelena, Radic Bozo, Filipovic Marinko, Blagaic Alenka Boban, Brcic Luka, Kolenc Danijela, Grabarevic Zeljko, Seiwerth Sven, Sikiric Predrag
Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, 10000 Zagreb, Croatia.
Department of Pharmacology and Toxicology, The Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000, Zagreb, Croatia.
Eur J Pharmacol. 2014 Mar 15;727:75-9. doi: 10.1016/j.ejphar.2014.01.046. Epub 2014 Jan 31.
Anesthetized mice or rats received intravenously 6%, 10%, 20%, 40%, 60%, 80%, and 90% dextran and/or white egg (1ml/rat or 0.15ml/mouse) into their tails. Medication (/kg b.w., 5ml/kg) was given intraperitoneally (BPC 157 10µg, 1µg, 10ng, and 10pg/kg, chloropyramine 20mg/kg, and cimetidine 10mg/kg intraperitoneally, alone or in combination while controls received an equivolume of saline), immediately after challenge or, alternatively, at 5min after or 24 or 48h before challenge. The effect was assessed at 5, 10, 20 and 30min after dextran and/or white egg challenge. We commonly noted prominent edema involving the face, upper and lower lip, snout, paws and scrotum (presented with extreme cyanosis), poor respiration and the number of fatalities after dextran and/or white egg application. Contrary, BPC 157 regimens (10µg, 1µg, 10ng, and 10pg/kg) effectively, may both prevent anaphylactoid reactions that may arise from dextran and/or white egg application and furthermore, rescue already advanced reactions when given after the challenge. Chloropyramine and cimetidine given alone were only moderately effective. When given together with BPC 157, the observed effect correlates with the strong effect of BPC 157 given alone.
将麻醉后的小鼠或大鼠通过尾静脉注射6%、10%、20%、40%、60%、80%和90%的右旋糖酐和/或白蛋白（1ml/大鼠或0.15ml/小鼠）。在激发后立即，或者在激发后5分钟、24小时或48小时之前，通过腹腔注射给药（/kg体重，5ml/kg）（BPC 157 10μg、1μg、10ng和10pg/kg，氯苯那敏20mg/kg，西咪替丁10mg/kg，单独或联合使用，而对照组接受等体积的生理盐水）。在右旋糖酐和/或白蛋白激发后5、10、20和30分钟评估效果。我们通常注意到，在应用右旋糖酐和/或白蛋白后，面部、上下唇、口鼻、爪子和阴囊出现明显水肿（伴有极度发绀）、呼吸急促和死亡数量。相反，BPC 157方案（10μg、1μg、10ng和10pg/kg）可以有效预防右旋糖酐和/或白蛋白应用可能引起的类过敏反应，此外，在激发后给药时还可以挽救已经进展的反应。单独使用氯苯那敏和西咪替丁效果中等。与BPC 157联合使用时，观察到的效果与单独使用BPC 157的强效效果相关。
