Macolino Christine M, Daiutolo Brittany V, Albertson Brad K, Elliott Melanie B
Department of Neurological Surgery, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA.
Jefferson Medical College, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA.
J Neurosci Methods. 2014 Apr 15;226:139-146. doi: 10.1016/j.jneumeth.2014.01.008. Epub 2014 Jan 31.
This study identifies the relationship between a test for post-traumatic headache and a marker for acute stress in rodent models of traumatic brain injury.
C57BL/6 mice and Sprague Dawley rats were divided into Controlled Cortical Impact (CCI) injury, craniotomy (CR), and incision groups. Periorbital and paw allodynia were evaluated using the von Frey test prior to injury and up to four weeks post-operatively. Serum corticosterone was evaluated in groups with and without mild restraint.
Periorbital and forepaw thresholds, but not hindpaw thresholds, were reduced in CCI and CR mice compared to incision (p<0.0001 and p<0.01). In contrast to mice, reduced periorbital and forepaw periorbital thresholds were found in CCI rats but not CR rats compared to incision (p<0.0001). Right periorbital thresholds were reduced compared to left thresholds for both rat and mouse at one week (p<0.01), but there were no side differences for forepaw thresholds. Hindpaw thresholds did not change from baseline values for any groups of mice or rats. In mice serum corticosterone levels were increased at one, two and four weeks post-CCI and CR, while the levels for rats were not different from incision (p<0.0001). Corticosterone levels were not different in mice subjected to restraint compared to no restraint.
This study presents novel data for allodynia in a rat model of TBI, and differences among mouse and rat species.
Mechanical allodynia occurs independent of evoked restraint stress, while hypothalamic pituitary adrenal axis activity is dependent on head trauma and species.
本研究确定了创伤性脑损伤啮齿动物模型中创伤后头痛测试与急性应激标志物之间的关系。
将C57BL/6小鼠和Sprague Dawley大鼠分为控制性皮质撞击（CCI）损伤组、开颅手术（CR）组和假手术组。在损伤前及术后长达四周的时间内，使用von Frey测试评估眶周和爪部的痛觉过敏情况。对有或无轻度束缚的组进行血清皮质酮评估。
与假手术组相比，CCI和CR小鼠的眶周和前爪阈值降低，但后爪阈值未降低（p<0.0001和p<0.01）。与小鼠不同，与假手术组相比，CCI大鼠的眶周和前爪眶周阈值降低，但CR大鼠未降低（p<0.0001）。在一周时，大鼠和小鼠的右侧眶周阈值均低于左侧（p<0.01），但前爪阈值无侧别差异。任何小鼠或大鼠组的后爪阈值均未偏离基线值。在小鼠中，CCI和CR术后1周、2周和4周血清皮质酮水平升高，而大鼠的水平与假手术组无差异（p<0.0001）。与无束缚的小鼠相比，受束缚小鼠的皮质酮水平无差异。
本研究提供了创伤性脑损伤大鼠模型中痛觉过敏的新数据，以及小鼠和大鼠物种之间的差异。
机械性痛觉过敏独立于诱发的束缚应激而发生，而下丘脑-垂体-肾上腺轴活动则取决于头部创伤和物种。
