Programa de Pós-Graduação em Neurociências, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil; Laboratório de Neurobiologia da Dor e Inflamação, Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Programa de Pós-Graduação em Neurociências, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil; Laboratório de Bioquímica do Exercício, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
J Neurosci Methods. 2018 Sep 1;307:23-30. doi: 10.1016/j.jneumeth.2018.06.004. Epub 2018 Jun 13.
This study demonstrated the effects of traumatic brain injury (TBI) and each step of the surgical procedure for a fluid percussion injury (FPI) model on periorbital allodynia.
Adult male Wistar rats were divided in naive, incision, scraping, sham-TBI and TBI groups. Periorbital allodynia was evaluated using von Frey filaments, and heat hyperalgesia of the hindpaws was evaluated by a Plantar Test Apparatus.
The statistical analyses revealed that the surgical procedure decreased von Frey filaments thresholds twenty-four hours after the surgery in all groups when compared to the naive group (p < 0.0001). Scraping, sham-TBI and TBI groups showed a decrease in the periorbital mechanical threshold for 35 days compared with the naive and incision groups (p < 0.0001). Only the TBI group demonstrated a significant difference in periorbital allodynia at 45 and 60 days after the injury (p < 0.01). A significant decrease in the thermal withdrawal latency of the hindpaw contralateral to the lesion was observed in the TBI group compared with the naive group at 7 days and 28 days after the lesion (p < 0.05).
This study presented in detail the effects of each stage of the surgical procedure for a FPI model on periorbital allodynia over time and characterized the TBI model for this evaluation.
The FPI model is relevant for the study of headache and generalized pain in both acute and chronic phases after an injury.
本研究旨在探讨创伤性脑损伤（TBI）以及流体冲击伤（FPI）模型手术各步骤对眶周触诱发痛的影响。
成年雄性 Wistar 大鼠分为未处理组、切口组、刮擦组、假手术 TBI 组和 TBI 组。眶周触诱发痛通过 von Frey 细丝进行评估，后肢热痛觉过敏通过足底测试仪器进行评估。
统计分析显示，与未处理组相比，手术二十四小时后所有组的 von Frey 细丝阈值均降低（p<0.0001）。与未处理组和切口组相比，刮擦组、假手术 TBI 组和 TBI 组在 35 天内眶周机械阈值降低（p<0.0001）。仅 TBI 组在损伤后 45 天和 60 天出现眶周触诱发痛的显著差异（p<0.01）。与未处理组相比，TBI 组在损伤后 7 天和 28 天，损伤对侧后肢热缩腿潜伏期显著降低（p<0.05）。
本研究详细介绍了 FPI 模型手术各阶段对眶周触诱发痛的影响，并对该评估的 TBI 模型进行了特征描述。
FPI 模型对于研究损伤后急性和慢性阶段的头痛和全身性疼痛具有重要意义。
