Murdock Courtney C, Blanford Simon, Hughes Grant L, Rasgon Jason L, Thomas Matthew B
Center for Infectious Disease Dynamics and Department of Entomology, Pennsylvania State University, University Park, PA, USA.
Sci Rep. 2014 Feb 3;4:3932. doi: 10.1038/srep03932.
Very recently, the Asian malaria vector (Anopheles stephensi) was stably transinfected with the wAlbB strain of Wolbachia, inducing refractoriness to the human malaria parasite Plasmodium falciparum. However, conditions in the field can differ substantially from those in the laboratory. We use the rodent malaria P. yoelii, and somatically transinfected An. stephensi as a model system to investigate whether the transmission blocking potential of wAlbB is likely to be robust across different thermal environments. wAlbB reduced malaria parasite prevalence and oocyst intensity at 28°C. At 24°C there was no effect on prevalence but a marked increase in oocyst intensity. At 20°C, wAlbB had no effect on prevalence or intensity. Additionally, we identified a novel effect of wAlbB that resulted in reduced sporozoite development across temperatures, counterbalancing the oocyst enhancement at 24°C. Our results demonstrate complex effects of temperature on the Wolbachia-malaria interaction, and suggest the impacts of transinfection might vary across diverse environments.
最近，亚洲疟疾媒介（斯氏按蚊）被沃尔巴克氏体的wAlbB菌株稳定转染，从而对人类疟原虫恶性疟原虫产生了抗性。然而，野外条件可能与实验室条件有很大差异。我们使用啮齿动物疟疾约氏疟原虫以及经体细胞转染的斯氏按蚊作为模型系统，来研究wAlbB的传播阻断潜力在不同热环境下是否可能具有稳健性。wAlbB在28°C时降低了疟原虫的流行率和卵囊强度。在24°C时，对流行率没有影响，但卵囊强度显著增加。在20°C时，wAlbB对流行率或强度均无影响。此外，我们发现了wAlbB的一种新效应，即导致跨温度条件下子孢子发育减少，抵消了24°C时卵囊的增加。我们的结果证明了温度对沃尔巴克氏体与疟疾相互作用的复杂影响，并表明转染的影响在不同环境中可能有所不同。
