Jinno Nobuko, Nagata Masashi, Takahashi Takeshi
Food Science Research Laboratories, Meiji Co., Ltd., 540 Naruda, Odawara City, Kanagawa, 250-0862, Japan,
Biol Trace Elem Res. 2014 Apr;158(1):65-72. doi: 10.1007/s12011-014-9901-2. Epub 2014 Feb 4.
The aim of the present study was to elucidate whether the recovery from muscle injury is impaired in marginal zinc deficiency. C57BL/6 male mice were fed a marginally zinc-deficient diet (MZD: 8 mg Zn/kg diet), a zinc-adequate diet (ZA: zinc 35 mg Zn/kg diet), and a zinc-high diet (ZH: 190 mg Zn/kg diet) for 4 weeks. Muscle injury was induced in the gastrocnemius muscles using cardiotoxin. The gastrocnemius muscles of these mice were harvested at 3, 5, 7, 10, 14, and 20 days after injury. We evaluated the regeneration of the skeletal muscle with hematoxylin and eosin staining and developmental myosin heavy-chain (dMHC: implicated in regeneration) immunostaining. The rate of dMHC-positive cells was significantly low in MZD mice compared with ZA mice at 3 days after cardiotoxin injection. The peak dMHC expression was found at 3 days after injection in ZA mice, 5 days in ZH mice, and 7 days in MZD mice. These results suggest that recovery from muscle injury might be partly impaired and delayed in MZD mice. Therefore, we strongly suggest the appropriate zinc intake to prevent the impairment of skeletal muscle regeneration.
本研究的目的是阐明边缘性锌缺乏是否会损害肌肉损伤后的恢复。将C57BL/6雄性小鼠分别喂食边缘性锌缺乏饮食（MZD：8毫克锌/千克饮食）、锌充足饮食（ZA：35毫克锌/千克饮食）和高锌饮食（ZH：190毫克锌/千克饮食）4周。使用心脏毒素诱导腓肠肌损伤。在损伤后3、5、7、10、14和20天采集这些小鼠的腓肠肌。我们用苏木精和伊红染色以及发育性肌球蛋白重链（dMHC：与再生有关）免疫染色评估骨骼肌的再生。在注射心脏毒素后3天，MZD小鼠中dMHC阳性细胞的比例与ZA小鼠相比显著较低。在ZA小鼠中，注射后3天发现dMHC表达达到峰值，在ZH小鼠中为5天，在MZD小鼠中为7天。这些结果表明，MZD小鼠的肌肉损伤恢复可能会部分受损并延迟。因此，我们强烈建议适当摄入锌以防止骨骼肌再生受损。
