Czerwinska Areta M, Streminska Wladyslawa, Ciemerych Maria A, Grabowska Iwona
Department of Cytology, Institute of Zoology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
Folia Histochem Cytobiol. 2012 Apr 24;50(1):144-53. doi: 10.2478/18710.
The goal of our study was to compare the skeletal muscle regeneration induced by two types of injury: either crushing, that causes muscle degeneration as a result of mechanical devastation of myofibers, or the injection of a cardiotoxin that is a myotoxic agent causing myolysis of myofibers leading to muscle degeneration. Regenerating muscles were analyzed at selected intervals, until the 14th day following the injury. We analyzed their weight and morphology. We also studied the expression of different myosin heavy chain isoforms as a molecular marker of the regeneration progress. Histological analysis revealed that inflammatory response and myotube formation in crushed muscles was delayed compared to cardiotoxin-injected ones. Moreover, the expression of myosin heavy chain isoforms was observed earlier in cardiotoxin-injured versus crushed muscles. We conclude that the dynamics of skeletal muscle regeneration depends on the method of injury.
一种是挤压伤，它会因肌纤维的机械性破坏而导致肌肉退化；另一种是注射心脏毒素，这是一种肌毒性剂，会导致肌纤维的肌溶解从而引起肌肉退化。在选定的时间间隔对再生肌肉进行分析，直至损伤后的第14天。我们分析了它们的重量和形态。我们还研究了不同肌球蛋白重链异构体的表达情况，以此作为再生进程的分子标志物。组织学分析显示，与注射心脏毒素的肌肉相比，挤压伤肌肉中的炎症反应和肌管形成有所延迟。此外，在注射心脏毒素损伤的肌肉中，肌球蛋白重链异构体的表达比挤压伤肌肉中更早被观察到。我们得出结论，骨骼肌再生的动态过程取决于损伤方法。
