Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada.
Planta. 1971 Mar;101(1):88-90. doi: 10.1007/BF00387693.
Cotyledons of cucumber plants grown in the dark for 7 days were treated with various concentrations of cytokinins for 14 h and then moved into light. After 3 h the treated cotyledons had up to 450% more chlorophyll than the water controls. This suggests that cytokinins have an important role in the formation of chlorophyll. The increase in chlorophyll level was proportional to cytokinin concentration and was apparent at concentrations as low as 0.001 mg/l. Sensitivity to cytokinins depended on the age of the cotyledons and the time of exposure to light. Gibberellic acid, indoleacetic acid, adenine and sucrose did not cause a similar increase in chlorophyll levels. This effect of cytokinins on chlorophyll formation is valuable as a simple, rapid bioassay for cytokinins.
将黑暗中生长 7 天的黄瓜植株子叶用不同浓度的细胞分裂素处理 14 小时，然后转移到光照下。3 小时后，处理过的子叶中的叶绿素含量比水对照组高出 450%。这表明细胞分裂素有在叶绿素形成过程中起重要作用。叶绿素水平的增加与细胞分裂素浓度成正比，即使在低至 0.001mg/L 的浓度下也很明显。对细胞分裂素的敏感性取决于子叶的年龄和暴露在光下的时间。赤霉素、吲哚乙酸、腺嘌呤和蔗糖不会导致叶绿素水平的类似增加。细胞分裂素对叶绿素形成的这种影响是有价值的，因为它是一种简单、快速的细胞分裂素生物测定法。
