Darah I, Tong W Y, Nor-Afifah S, Nurul-Aili Z, Lim S H
Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences University Sains Malaysia, Penang, Malaysia.
Eur Rev Med Pharmacol Sci. 2014;18(2):171-8.
Caulerpa (C.) sertularioides has many therapeutic uses in the practice of traditional medicine in Malaysia. Crude methanolic, diethyl ether extract, ethyl acetate extract and butanolic extract from C. sertularioides were subjected to antimicrobial screening including the three Gram-positive and three Gram-negative diarrhea-caused bacteria.
The antimicrobial activities were studied by using disc diffusion method and broth dilution method. The effect of the extract on the growth profile of the bacteria was examined via time-kill assay. In addition to the bactericidal effects study, microscopic observations using Scanning electron microscopy (SEM) was done to determine the major alterations in the microstructure of Bacillus (B.) subtilis.
Ethyl acetate extract demonstrated antibacterial activity towards all the tested bacteria and produced inhibition zone ranging from ≤ 9 mm - ≥ 15 mm. However, all the tested bacteria were resistant to the butanolic extract treatment. B. subtilis growth curve in the presence of the crude methanol extract at MIC showed bacteriostatic. The main abnormalities found from these microscopic observations were morphology alteration of the bacteria cells after exposure to the methanol extract.
Data from this study revealed that C. sertularioides may be potential antimicrobial agents against foodborne Gram-positive and Gram-negative bacteria particularly cause diarrhea, and also food spoilage microorganisms.
在马来西亚的传统医学实践中，蕨藻具有多种治疗用途。对蕨藻的甲醇粗提物、乙醚提取物、乙酸乙酯提取物和丁醇提取物进行了抗菌筛选，受试细菌包括三种革兰氏阳性和三种革兰氏阴性腹泻致病菌。
采用纸片扩散法和肉汤稀释法研究抗菌活性。通过时间杀菌试验检测提取物对细菌生长曲线的影响。除了研究杀菌效果外，还利用扫描电子显微镜（SEM）进行微观观察，以确定枯草芽孢杆菌微观结构的主要变化。
乙酸乙酯提取物对所有受试细菌均表现出抗菌活性，抑菌圈范围为≤9毫米至≥15毫米。然而，所有受试细菌对丁醇提取物处理均有抗性。在最低抑菌浓度（MIC）下，枯草芽孢杆菌在粗甲醇提取物存在时的生长曲线显示为抑菌。这些微观观察发现的主要异常情况是细菌细胞在接触甲醇提取物后形态发生改变。
本研究数据表明，蕨藻可能是对抗食源性革兰氏阳性和革兰氏阴性细菌（特别是引起腹泻的细菌）以及食品腐败微生物的潜在抗菌剂。
