He He, Lü Rui-Xue, Xu Jin, Liang Li-Bo, Zhang Ming-Feng, An Zhen-Mei, Huang Heng-Jian
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Department of General Practice Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2013 Nov;44(6):920-3.
To preliminarily investigate the expression of cysteine proteinase inhibitors C (cystatin C) in primary hepatic carcinoma.
Hepatic tissue samples and peripheral blood samples were collected from 41 cases of primary hepatic carcinoma, 24 cases of cirrhosis and 40 cases of normal control. To primary hepatic carcinoma, three kinds of hepatic tissue samples were harvested, including carcinoma tissue, adjacent non-tumor tissue, and distant normal tissue. The expression levels of cystatin C in hepatic samples and blood samples were measured by immunohistochemistry method and latex enhanced immune turbidimetric method respectively, and the differences of cystatin C expressions were compared in primary hepatic cancer, cirrhosis, and normal control. Furthermore, the relationships of cystatin C expression with tumor size, intrahepatic metastasis, serum AFP level were studied with correlation analysis.
The expression of cystatin C was positive, in primary hepatic carcinoma. Part of adjacent non-tumor tissue, and distant normal tissue and cirrhosis tissues had some degree of cyctatin C expression. Wilcoxon test showed that the differences of cyctatin C expression in different hepatic tissues were statistics significance (P < 0.01). The proportion of positive cell, staining intensity, and the product of these two was: carcinoma tissues > adjacent non-tumor tissues> cirrhosis tissues > distant normal tissue. Compared with normal control, primary hepatic carcinoma and cirrhosis both had higher serum cystatin C level (P < 0.001), but there was no difference between hepatic carcinoma and cirrhosis (P = 0.769). The correlation analysis showed that the level of serum cystatin C was not related to serum AFP, tumor size, or intrahepatic metastasis.
The expression of cystatin C in primary hepatic carcinoma is higher than that of adjacent non-tumor tissue, distant normal tissue, and cirrohsis tissue. The serum cystatin C level of primary hepatic carcinoma is higher than that of normal control.
初步探讨半胱氨酸蛋白酶抑制剂C（胱抑素C）在原发性肝癌中的表达情况。
收集41例原发性肝癌患者、24例肝硬化患者及40例正常对照者的肝组织样本和外周血样本。对于原发性肝癌患者，采集三种肝组织样本，包括癌组织、癌旁非肿瘤组织和远处正常组织。分别采用免疫组织化学法和乳胶增强免疫比浊法检测肝组织样本和血液样本中胱抑素C的表达水平，并比较原发性肝癌、肝硬化和正常对照中胱抑素C表达的差异。此外，通过相关性分析研究胱抑素C表达与肿瘤大小、肝内转移、血清甲胎蛋白水平的关系。
原发性肝癌中胱抑素C表达呈阳性。部分癌旁非肿瘤组织、远处正常组织及肝硬化组织有一定程度的胱抑素C表达。Wilcoxon检验显示，不同肝组织中胱抑素C表达差异具有统计学意义（P<0.01）。阳性细胞比例、染色强度及其乘积为：癌组织>癌旁非肿瘤组织>肝硬化组织>远处正常组织。与正常对照相比，原发性肝癌和肝硬化患者血清胱抑素C水平均较高（P<0.001），但肝癌与肝硬化之间无差异（P=0.769）。相关性分析显示，血清胱抑素C水平与血清甲胎蛋白、肿瘤大小或肝内转移无关。
原发性肝癌中胱抑素C的表达高于癌旁非肿瘤组织、远处正常组织及肝硬化组织。原发性肝癌患者血清胱抑素C水平高于正常对照。
