南极磷虾（Euphausia superba）多态微卫星位点的特征分析
Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba.
作者信息
Candeias Rui, Teixeira Sara, Duarte Carlos M, Pearson Gareth A
机构信息
Centre of Marine Sciences, CCMAR-CIMAR, University of the Algarve, Gambelas Campus, 8005-139 Faro, Portugal.
出版信息
BMC Res Notes. 2014 Feb 3;7:73. doi: 10.1186/1756-0500-7-73.
BACKGROUND
The Antarctic krill, Euphausia superba is a pelagic crustacean, abundant in high-density swarms (10,000 - 30,000 ind/m2) with a circumpolar distribution and a key role in the food web of the Southern Ocean. Only three EST derived microsatellite markers have been used in previous genetic studies, hence we developed additional highly polymorphic microsatellite markers to allow robust studies of the genetic variability and population differentiation within this species.
FINDINGS
The microsatellite markers described here were obtained through an enriched genomic library, followed by 454 pyrosequencing. A total of 10 microsatellite markers were tested in 32 individuals from the Antarctic Peninsula. One of the tested loci was fixed for one allele while the other was variable. Of the remaining nine markers, seven showed no departure from Hardy-Weinberg equilibrium. The mean number of alleles was 14.9.
CONCLUSIONS
These markers open perspectives for population genetic studies of this species to unravel genetic structure, dispersal and population biology, vital information for future conservation.
背景
南极磷虾（Euphausia superba）是一种浮游甲壳类动物，在高密度群体（10,000 - 30,000 只/平方米）中数量丰富，分布于环南极地区，在南大洋食物网中起关键作用。在以往的遗传研究中仅使用了三个源自 EST 的微卫星标记，因此我们开发了更多高度多态性的微卫星标记，以便对该物种的遗传变异性和种群分化进行可靠研究。
研究结果
此处描述的微卫星标记是通过富集基因组文库，随后进行 454 焦磷酸测序获得的。在来自南极半岛的 32 个个体中对总共 10 个微卫星标记进行了测试。其中一个测试位点的一个等位基因固定，另一个可变。在其余九个标记中，七个未偏离哈迪 - 温伯格平衡。等位基因的平均数量为 14.9。
结论
这些标记为该物种的种群遗传学研究开辟了前景，以揭示遗传结构、扩散和种群生物学，这些是未来保护的重要信息。
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