Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Laboratory of Phytochemistry and Medicinal Chemistry, Department of Pharmacy, Alfenas Federal University, Alfenas, Minas Gerais, Brazil.
J Photochem Photobiol B. 2014 Feb 5;131:65-73. doi: 10.1016/j.jphotobiol.2014.01.004. Epub 2014 Jan 17.
The damaging effects of sunlight to the skin has triggered studies that involve the synthesis and extraction of organic compounds from natural sources that can absorb UV radiation, and studies on polyphenolic compounds with antioxidant and anti-inflammatory properties that can be used as photochemopreventive agents for reducing skin damage. We investigated the in vitro and in vivo photoprotective/photochemopreventive potential of Garcinia brasiliensis epicarp extract (GbEE). We evaluated the cell viability of L929 fibroblasts after UVB exposure using a quartz plate containing the extract solution or the GbEE formulation. The in vivo photoprotective effect of the GbEE formulation was evaluated by measuring the UVB damage-induced decrease in endogenous reduced glutathione (GSH), the increase in myeloperoxidase (MPO) activity and secretion of cytokines IL-1β and TNF-α. The in vitro methodology using fibroblasts showed that the photoprotective properties of the GbEE solutions and 10% GbEE formulation were similar to the commercial sunscreen (SPF-15). In vivo results demonstrated of the GbEE formulation in decreasing UVB induced-damage such as GSH depletion, an increased in MPO activity and secretion of cytokines IL-1β and TNF-α. The results showed that the extract has great potential for use as a sunscreen in topical formulations in addition to UV filters.
阳光对皮肤的有害影响引发了研究,这些研究涉及从天然来源合成和提取可吸收紫外线辐射的有机化合物,以及研究具有抗氧化和抗炎特性的多酚化合物,这些化合物可用作减少皮肤损伤的光化学预防剂。我们研究了藤黄果皮提取物(GbEE)的体外和体内光保护/光化学预防潜力。我们使用含有提取物溶液或 GbEE 制剂的石英板评估了 L929 成纤维细胞在 UVB 暴露后的细胞活力。通过测量 UVB 损伤诱导的内源性还原型谷胱甘肽(GSH)减少、髓过氧化物酶(MPO)活性增加和细胞因子 IL-1β 和 TNF-α的分泌,评估了 GbEE 制剂的体内光保护作用。使用成纤维细胞的体外方法表明,GbEE 溶液和 10%GbEE 制剂的光保护特性与商业防晒霜(SPF-15)相似。体内结果表明 GbEE 制剂可减少 UVB 诱导的损伤,如 GSH 耗竭、MPO 活性增加和细胞因子 IL-1β 和 TNF-α的分泌。结果表明,该提取物除了紫外线滤光片外,还有很大的潜力用作局部制剂中的防晒霜。