Bio-Evaluation Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Cheongwon, 363-883, Republic of Korea.
Life Sciences Research Institute, Biomedic Co., Ltd., Bucheon 420-020, Republic of Korea.
Food Chem. 2014 Jun 15;153:145-50. doi: 10.1016/j.foodchem.2013.12.051. Epub 2013 Dec 16.
Comparing well-watered versus deficit conditions, we evaluated the chemical composition of grains harvested from wild-type (WT) and drought-tolerant, transgenic rice (Oryza sativa L.). The latter had been developed by inserting AtCYP78A7, which encodes a cytochrome P450 protein. Two transgenic Lines, '10B-5' and '18A-4', and the 'Hwayoung' WT were grown under a rainout shelter. After the harvested grains were polished, their levels of key components, including proximates, amino acids, fatty acids, minerals and vitamins were analysed to determine the effect of watering system and genotype. Drought treatment significantly influenced the levels of some nutritional components in both transgenic and WT grains. In particular, the amounts of lignoceric acid and copper in the WT decreased by 12.6% and 39.5%, respectively, by drought stress, whereas those of copper and potassium in the transgenics rose by 88.1-113.3% and 10.4-11.9%, respectively, under water-deficit conditions.
在对比水分充足和水分亏缺条件下,我们评估了从野生型(WT)和耐旱转基因水稻(Oryza sativa L.)中收获的谷物的化学成分。后者是通过插入编码细胞色素 P450 蛋白的 AtCYP78A7 开发的。两个转基因系'10B-5'和'18A-4'以及'Hwayoung'WT 在避雨棚下生长。收获的谷物经过抛光后,分析其关键成分(包括粗蛋白、氨基酸、脂肪酸、矿物质和维生素)的水平,以确定灌溉系统和基因型的影响。干旱处理显著影响了转基因和 WT 谷物中一些营养成分的水平。特别是,WT 中的愈创木酸和铜含量分别因干旱胁迫而下降了 12.6%和 39.5%,而在水分亏缺条件下,转基因中的铜和钾含量分别上升了 88.1-113.3%和 10.4-11.9%。