Analytical Chemistry Department, Faculty of Sciences, University of Burgos, Pza. Misael Bañuelos s/n, 09001 Burgos, Spain.
Analytical Chemistry Department, Faculty of Sciences, University of Burgos, Pza. Misael Bañuelos s/n, 09001 Burgos, Spain.
Anal Chim Acta. 2014 Feb 17;812:41-4. doi: 10.1016/j.aca.2013.12.041. Epub 2014 Jan 9.
Screen-printed carbon electrodes have been modified with tetrathiafulvalene and sulfite oxidase enzyme for the sensitive and selective detection of sulfite. Amperometric experimental conditions were optimized taking into account the importance of quantifying sulfite in wine samples and the inherent complexity of these samples, particularly red wine. The biosensor responds to sulfite giving a cathodic current (at +200 mV vs screen-printed Ag/AgCl electrode and pH 6) in a wide concentration range, with a capability of detection of 6 μM (α=β=0.05) at 60°C. The method has been applied to the determination of sulfite in white and red samples, with averages recoveries of 101.5% to 101.8%, respectively.
丝网印刷碳电极经过修饰，引入了四硫富瓦烯和亚硫酸氧化酶，用于对亚硫酸盐进行灵敏和选择性检测。在考虑到葡萄酒样品中亚硫酸盐定量的重要性以及这些样品固有复杂性的情况下，优化了电流测定实验条件，尤其是红酒。该生物传感器对亚硫酸盐有响应，在宽浓度范围内产生阴极电流（在+200 mV 对丝网印刷 Ag/AgCl 电极，pH 值为 6），在 60°C 时检测限为 6 μM（α=β=0.05）。该方法已应用于白葡萄酒和红葡萄酒中亚硫酸盐的测定，平均回收率分别为 101.5%和 101.8%。
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