使用配备紫外检测器的高效液相色谱法对非特应性成年人呼出的白三烯B4水平进行定量分析。

Using high-performance liquid chromatography with UV detector to quantify exhaled leukotriene B4 level in nonatopic adults.

作者信息

Wan Gwo-Hwa, Yan Dah-Chin, Tseng Hui-Yun, Lee Jian-Tao, Lin Yu-Wen

机构信息

Department of Respiratory Therapy, College of Medicine, Chang Gung University, Taoyuan, Taiwan.

Division of Taipei Pediatrics, Department of Pediatrics, Chang Gung Children's Hospital, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Department of Respiratory Therapy, Chang Gung Memorial Hospital, Taoyuan, Taiwan; College of Medicine, Chang Gung University, Taoyuan, Taiwan.

出版信息

J Formos Med Assoc. 2014 Aug;113(8):566-8. doi: 10.1016/j.jfma.2013.12.009. Epub 2014 Feb 1.

DOI:10.1016/j.jfma.2013.12.009

PMID:24491995

Abstract

This study aimed to evaluate the feasibility of the chemical method to analyze exhaled breath condensate (EBC) leukotriene B4 (LTB4) level in humans. High-performance liquid chromatography with a UV detector was applied to quantify the inflammatory biomarker. The LTB4 concentration in the concentrated pooled EBC samples was 1.19 ng/μL, and the average LTB4 concentration of each EBC sample was 15.38 ng/μL. This analytical technique was feasible to evaluate the levels of inflammatory mediators such as LTB4 in human EBCs without any complicated sample pretreatment processes.

摘要

本研究旨在评估化学方法分析人体呼出气冷凝液（EBC）中白三烯B4（LTB4）水平的可行性。采用配有紫外检测器的高效液相色谱法对这种炎症生物标志物进行定量分析。浓缩合并的EBC样本中LTB4浓度为1.19 ng/μL，每个EBC样本的平均LTB4浓度为15.38 ng/μL。这种分析技术可在无需任何复杂样本预处理过程的情况下，评估人体EBC中LTB4等炎症介质的水平。