Departamento de Biología Molecular, Área de Bioquímica y Biología Molecular, Universidad de León, Campus de Vegazana, 24071 León, Spain.
Departamento de Biología Molecular, Área de Bioquímica y Biología Molecular, Universidad de León, Campus de Vegazana, 24071 León, Spain.
FEBS Lett. 2014 Mar 18;588(6):922-8. doi: 10.1016/j.febslet.2014.01.047. Epub 2014 Jan 31.
The transcriptional antiterminator RfaH promotes transcription of long operons encoding surface cell components important for the virulence of Escherichiacoli pathogens. In this paper, we show that RfaH enhanced kps expression for the synthesis of group 2 polysialic acid capsule in E. coli K92. In addition, we demonstrate for the first time that RfaH promotes cps expression for the synthesis of colanic acid, a cell wall component with apparently no role on pathogenicity. Finally, we show a novel RfaH requirement for growth at low temperatures.
转录终止子拮抗剂 RfaH 促进编码表面细胞成分的长操纵子的转录，这些成分对大肠杆菌病原体的毒力很重要。在本文中，我们表明 RfaH 增强了 kps 的表达，以合成大肠杆菌 K92 中的 2 组多糖唾液酸荚膜。此外，我们首次证明 RfaH 促进了 cps 的表达，以合成细胞壁成分粘酸，该成分显然对致病性没有作用。最后，我们展示了 RfaH 在低温下生长的新需求。
