大豆叶绿体质子转运ATP酶亚基III编码基因的核苷酸序列及转录分析

Nucleotide sequence and transcription analysis of the gene coding for subunit III of soybean chloroplast proton-translocating ATPase.

作者信息

Wintz H, Skunca M, Pillay D T

机构信息

Department of Biological Sciences, University of Windsor, Ont., Canada.

出版信息

Gene. 1987;59(1):47-53. doi: 10.1016/0378-1119(87)90265-4.

DOI:10.1016/0378-1119(87)90265-4

PMID:2449380

Abstract

The gene coding for subunit III of CF0-ATPase complex (atpH gene) has been localized on the soybean chloroplast genome by heterologous hybridization with the atpH gene of wheat chloroplast. The gene, 243 bp in length, is located in a 7.5-kb BamHI fragment generated from the 14.8-kb PvuII fragment in the large single-copy region of the chloroplast genome. The nucleotide sequence of the gene has been determined. The deduced amino acid (aa) sequence shows 2 aa changes relative to its homologue from wheat and spinach and 1 aa with respect to that found in tobacco chloroplast. The gene is apparently transcribed independently as an 850-nt transcript.

摘要

通过与小麦叶绿体的atpH基因进行异源杂交，已将编码CF0-ATP酶复合体亚基III的基因（atpH基因）定位在大豆叶绿体基因组上。该基因长度为243 bp，位于叶绿体基因组大单拷贝区域中由14.8 kb PvuII片段产生的7.5 kb BamHI片段上。已确定该基因的核苷酸序列。推导的氨基酸序列与来自小麦和菠菜的同源物相比有2个氨基酸变化，与烟草叶绿体中的相比有1个氨基酸变化。该基因显然作为一个850 nt的转录本独立转录。