编码ATP合酶CFo亚基III的豌豆叶绿体基因的核苷酸序列及转录本。

Nucleotide sequence and transcripts of the pea chloroplast gene encoding CFo subunit III of ATP synthase.

作者信息

Huttly A K, Plant A L, Phillips A L, Auffret A D, Gray J C

机构信息

Botany School, University of Cambridge, U.K.

出版信息

Gene. 1990 Jun 15;90(2):227-33. doi: 10.1016/0378-1119(90)90184-s.

DOI:10.1016/0378-1119(90)90184-s

PMID:2129529

Abstract

The structure and expression of the pea chloroplast atpH gene, encoding ATP synthase CFo subunit III, have been investigated. The atpH gene is situated between the atpI and atpF genes for CFo subunits IV and I, and encodes a hydrophobic polypeptide of 81 amino acid residues which is very similar to subunit III from other species. Analysis of transcripts from the region of chloroplast DNA encoding ATP synthase subunits IV-III-I-alpha shows a complex pattern of transcription, with large transcripts potentially coding for several subunits and also smaller gene-specific transcripts. Two abundant transcripts of 660 nucleotides (nt) and 980 nt specific for atpH were identified. Primer extension and S1 nuclease protection mapping suggested that the 660-nt transcripts were produced by endonucleolytic processing at the sequence, 5'-UGGAAU.

摘要

对编码ATP合酶CFo亚基III的豌豆叶绿体atpH基因的结构和表达进行了研究。atpH基因位于CFo亚基IV和I的atpI和atpF基因之间，编码一个由81个氨基酸残基组成的疏水多肽，该多肽与其他物种的亚基III非常相似。对叶绿体DNA编码ATP合酶亚基IV-III-I-α区域的转录本分析显示出复杂的转录模式，有潜在编码几个亚基的大转录本以及较小的基因特异性转录本。鉴定出了两个660个核苷酸（nt）和980 nt的atpH特异性丰富转录本。引物延伸和S1核酸酶保护图谱分析表明，660-nt转录本是通过在5'-UGGAAU序列处进行内切核酸酶加工产生的。