Department of General Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Iran J Basic Med Sci. 2013 Nov;16(11):1151-4.
Breast cancer is the most common type of cancer among women worldwide. This study investigated the expression and clinical significance of activating transcription factor 3 (ATF3) in human breast cancer and its relationship with the clinical outcome of breast cancer. Materials and Methods : ATF3 expressions were detected in 114 primary breast cancer tissues and 114 adjacent normal tissues using immunohistochemistry (IHC) assay. Categorical variables were statistically compared by chi-square or Fisher's exact test. Survival curves were evaluated using the Kaplan-Meier method and comparisons of survival rates were tested using a Log-rank test. Results : IHC analysis showed that the positive expression of ATF3 protein was detected in breast cancer tissue with a positive ratio of 76.3%, and the positive ATF3 expression in adjacent normal breast tissue was 13.2%, which is lower than that in breast cancer tissue samples (P<0.01). Furthermore, ATF3 expression showed significant correlation with TNM stage, invasion, lymph node metastasis and number of metastatic lymph nodes (P=0.038, P=0.029, P=0.026, and P=0.039 respectively), and did not correlate with patients' age and tumor size (P>0.05). A significant difference in overall survival rate was found between the patients with positive expression of ATF3 protein and those with negative expression (P=0.041). Conclusion : Increased ATF3 expression participate in the tumorigenesis, invasion and metastasis of breast cancer, and ATF3 may be useful as a new prognostic indicator for breast cancer patients.
乳腺癌是全球女性最常见的癌症类型。本研究探讨了激活转录因子 3(ATF3)在人乳腺癌中的表达及其与乳腺癌临床结局的关系。
采用免疫组织化学(IHC)法检测 114 例原发性乳腺癌组织和 114 例相邻正常组织中 ATF3 的表达。采用卡方或 Fisher 确切检验比较分类变量。采用 Kaplan-Meier 法评估生存曲线,采用 Log-rank 检验比较生存率。
IHC 分析显示,乳腺癌组织中 ATF3 蛋白阳性表达率为 76.3%,而相邻正常乳腺组织中 ATF3 阳性表达率为 13.2%,低于乳腺癌组织样本(P<0.01)。此外,ATF3 表达与 TNM 分期、浸润、淋巴结转移和转移淋巴结数均呈显著相关(P=0.038、P=0.029、P=0.026、P=0.039),与患者年龄和肿瘤大小无关(P>0.05)。ATF3 蛋白阳性表达的患者总生存率明显低于阴性表达的患者(P=0.041)。
ATF3 表达增加参与乳腺癌的发生、浸润和转移,ATF3 可能成为乳腺癌患者新的预后指标。