瘦素受体 Q223R 多态性对乳腺癌风险的影响。
Effect of leptin receptor Q223R polymorphism on breast cancer risk.
机构信息
Hyperlipidemia Research Center, Department of Clinical Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Cellular and Molecular Research Center, Department of Clinical Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Iran J Basic Med Sci. 2014 Aug;17(8):588-94.
OBJECTIVES
Leptin receptor (LEPR) is a member of the class I cytokine receptor super-family that is known implicated in the initiation and progression of breast cancer. We have investigated the effect of Q223R polymorphism on the breast cancer susceptibly in a sample of Iranian subjects.
MATERIALS AND METHODS
We utilized a polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method to investigate the association of LEPR Q223R polymorphism with breast cancer risk in a case control study consisting of 100 breast cancer cases and 100 controls without breast cancer. Serum levels of leptin and soluble leptin receptor (sOB-R) were measured by ELISA method.
RESULTS
The genotype (QQ, QR, and RR) distributions were 25, 56, and 19 % in breast cancer cases and 54, 40, and 6% in controls, respectively. The frequency of 223 RR genotype was significantly elevated in breast cancer cases as compared to controls (χ(2)= 20.072, P<0.001). Similar significance differences were also found in allele frequencies for Q and R between two groups (χ(2)= 19.027, P< 0.001). Additionally, there were significant association between Q223R genotypes and breast cancer risk; homozygotes for RR genotype (OR= 6.840; 95% confidence interval [CI] = 2.434-19.218), heterozygotes for QR (OR=3.024; 95% CI = 1.620-5.644, P = 0.001), and QR+RR genotype (OR= 3.522; 95% CI = 1.934-6.414, P < 0.001), respectively.
CONCLUSION
Our results showed that the LEPR Q223R polymorphism is associated with increased breast cancer risk as well as tumor grade in a sample of Iranian subjects.
目的
瘦素受体（LEPR）是细胞因子受体超家族 I 类的成员，已知其参与乳腺癌的发生和发展。我们研究了 Q223R 多态性对伊朗人群乳腺癌易感性的影响。
材料和方法
我们采用聚合酶链反应（PCR）限制性片段长度多态性（RFLP）方法，在病例对照研究中调查了 LEPR Q223R 多态性与乳腺癌风险的关联，该研究包括 100 例乳腺癌病例和 100 例无乳腺癌对照。采用 ELISA 法测定血清瘦素和可溶性瘦素受体（sOB-R）水平。
结果
乳腺癌病例组的基因型（QQ、QR 和 RR）分布分别为 25%、56%和 19%，对照组分别为 54%、40%和 6%。与对照组相比，乳腺癌病例组 223RR 基因型的频率显著升高（χ²=20.072，P<0.001）。两组间 Q 和 R 等位基因频率也存在显著差异（χ²=19.027，P<0.001）。此外，Q223R 基因型与乳腺癌风险之间存在显著关联；RR 基因型纯合子（OR=6.840；95%置信区间[CI] =2.434-19.218）、QR 杂合子（OR=3.024；95% CI =1.620-5.644，P=0.001）和 QR+RR 基因型（OR=3.522；95% CI =1.934-6.414，P<0.001）。
结论
我们的研究结果表明，在伊朗人群中，LEPR Q223R 多态性与乳腺癌风险增加以及肿瘤分级有关。
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