Yang Zong-Bao, Wang Chen-Guang, Gong An, Xie Yu-feng, Liu Qiong, Yang Qing
Department of TCM, Medical College of Xiamen University, Xiamen 361005, Fujian Province, China.
Jiangxi University of TCM, China.
Zhongguo Zhen Jiu. 2013 Nov;33(11):1017-21.
To explore relevant material basis of moxibustion for recovering gastric mucosal lesion. METHODL Forty-five SD rats were randomly divided into a normal goup, a model group, an acupoint group and a control group, 15 rats in the model group and 10 rats in the rest three groups. Except the normal group, binding and cold stress method were used to establish gastric mucosa injury model. The suspended moxibustion was applied in the acupoint group and control group at acupoints of the stomach meridian ("Liangmen" (ST 21) and "Zusanli" (ST36) and control acupoints (Laterally 1cm next to the "Liangmen" (ST 21) and Zusanli" (ST36), once a day, consectutively for 12 days. After 12 days, morphology of gastric mucosal was observed under optical microscope; protein fingerprints of gastric mucosa cell in rats were detected by protein fingerprint technology, weak cation chip and weak anion chip. Also mass to charge ratio of differential proteins in groups were compared and analyzed.
Compared with the model group, index of gastric mucosal lesion in the acupoint group was reduced and its morphology was obviously improved (P<0.05). Campared with control group, index and morphology of gastric mucosal lesion were significantly improved in the acupoint group (P<0.05). According to test of weak cation chip, there was four marker proteins that had expression differences, indicating moxibustion at acupoints of stomach meridian could inrease expression of three marker protein whose molecular weight was 1354Da, 5692Da and 8432Da (all P<0.05) while reduce expression of marker protein with molecular weight of 3287Da (_<0.05). According to test of weak anion chip, moxibustion at acupoints of stomach meridian could increase expression of three marker proteins whose molecular weight was 2412 Da, 3026Da and 6475 Da (allP<0.05).
Moxibustion at acupoints of the stomach meridian could regulate differential expression of gastric mucosa cell-related marker protein in rats with acute gastric ulcer and recover gastric mucosal lesion, it's effect is better than that of the points of laterally 1 cm next to acupoint.
探讨艾灸修复胃黏膜损伤的相关物质基础。方法：将45只SD大鼠随机分为正常组、模型组、穴位组和对照组，模型组15只，其余3组各10只。除正常组外，采用束缚加寒冷应激法建立胃黏膜损伤模型。穴位组和对照组采用悬灸胃经穴位（“梁门”（ST21）和“足三里”（ST36））及对照穴位（“梁门”（ST21）和“足三里”（ST36）旁开1cm处），每日1次，连续12天。12天后，在光学显微镜下观察胃黏膜形态；采用蛋白质指纹技术、弱阳离子芯片和弱阴离子芯片检测大鼠胃黏膜细胞的蛋白质指纹图谱。同时比较分析各组差异蛋白的质荷比。
与模型组比较，穴位组胃黏膜损伤指数降低，形态明显改善（P<0.05）。与对照组比较，穴位组胃黏膜损伤指数及形态均明显改善（P<0.05）。弱阳离子芯片检测显示，有4种标志物蛋白表达存在差异，提示艾灸胃经穴位可使分子量为1354Da、5692Da和8432Da的3种标志物蛋白表达增加（均P<0.05），而使分子量为3287Da的标志物蛋白表达降低（P<0.05）。弱阴离子芯片检测显示，艾灸胃经穴位可使分子量为2412Da、3026Da和6475Da的3种标志物蛋白表达增加（均P<0.05）。
艾灸胃经穴位可调节急性胃溃疡大鼠胃黏膜细胞相关标志物蛋白的差异表达，修复胃黏膜损伤，其效果优于穴位旁开1cm处对照点。
