Peng Li, Wang Yadong, Chang Xiaorong, Wu Huangan, Liu Mi, Wang Hong, Chen Jiaolong, Quan Renfu, Yang Zongbao
J Tradit Chin Med. 2016 Jun;36(3):340-6. doi: 10.1016/s0254-6272(16)30047-4.
To observe the effect of moxa-burning heat stimulating acupoints of Liangmen (ST 21) and Zusanli (ST 36) on the proliferation and apoptosis signaling proteins in rats with stress-induced gastric ulcer.
Forty rats were randomly divided into four groups: negative control (NC), ulcer control (UC), acupoints of stomach meridian (ASM), and acupoints control (AC). The acute gastric ulcer model was established by bound and water immersion. Rats in NC and UC groups didn't receive any moxa-burning heat stimulating treatment, while rats in ASM and AC groups were treated with buringmoxa heat stimulating the acupoints of Liangmen (ST 21) and Zusanli (ST 36) and their controlled points, respectively. Rats in all groups were sacrificed after 12 consecutive days treatment. The ulcer index was evaluated by using Guth's method. The expression of tumor necrosis factor-alpha (TNF-α), apoptotic protease activating facter-1 (Apaf-1), Caspase-3, p21 activated kinase 1 (PAK1), extracellular regulated protein kinases 2 (ERK2), phosphorylated ERK2 (pERK2), phosphoinositide 3-kinase (PI3K) and RAC-alpha serine/threonine-protein kinase (Akt) in gastric mucosa was detected by enzyme linked immunosorbent assay (ELISA).
Compared with UC group, the ulcer index of ASM and AC groups decreased, and the injured gastric mucosa was improved, the expression of TNF-α, Apaf-1 and Caspase-3 in gastric mucosa was significantly reduced (P < 0.05), while the expression of PAK1, ERK2, pERK2, PI3K and Akt in gastric mucosa was significantly increased (P < 0.05). And ASM showed better effect than AC group (P < 0.05).
Moxa-burning Heat stimulating of Liangmen (ST 21) and Zusanli (ST 36) could promote the recovery of gastric mucosal lesion probably by inhibiting cell apoptosis and promoting cell proliferation in stress-induced gastric ulcer.
观察艾灸梁门（ST21）、足三里（ST36）穴对应激性胃溃疡大鼠增殖和凋亡信号蛋白的影响。
将40只大鼠随机分为四组：阴性对照组（NC）、溃疡对照组（UC）、胃经穴位组（ASM）和穴位对照组（AC）。采用束缚水浸法建立急性胃溃疡模型。NC组和UC组大鼠未接受任何艾灸温热刺激治疗，而ASM组和AC组大鼠分别给予艾灸温热刺激梁门（ST21）、足三里（ST36）穴及其对照点。连续治疗12天后，处死所有组大鼠。采用Guth法评估溃疡指数。通过酶联免疫吸附测定（ELISA）检测胃黏膜中肿瘤坏死因子-α（TNF-α）、凋亡蛋白酶激活因子-1（Apaf-1）、半胱天冬酶-3（Caspase-3）、p21激活激酶1（PAK1）、细胞外调节蛋白激酶2（ERK2）、磷酸化ERK2（pERK2）、磷脂酰肌醇3激酶（PI３K）和RAC-α丝氨酸/苏氨酸蛋白激酶（Akt）的表达。
与UC组相比，ASM组和AC组溃疡指数降低，胃黏膜损伤改善，胃黏膜中TNF-α、Apaf-1和Caspase-3的表达显著降低（P<0.05），而胃黏膜中PAK1、ERK2、pERK2、PI3K和Akt的表达显著增加（P<0.05）。且ASM组效果优于AC组（P<0.05）。
艾灸梁门（ST21）、足三里（ST36）穴可能通过抑制应激性胃溃疡大鼠细胞凋亡、促进细胞增殖，从而促进胃黏膜损伤的修复。
