Ruan Yu, Qian Wei-ping, Zhang Chun-hui, Zhou Liang, Hou Zhen-hui
Department of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen 518036, China.
Department of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen 518036, China. Email:
Zhonghua Fu Chan Ke Za Zhi. 2013 Dec;48(12):907-10.
To study the different expression of microRNA(miRNA) including mir-29c, mir-200a, mir-145 in the mid-secretary endometrium and its relationship with infertility of endometriosis.
From August 2011 to February 2013, 36 infertile cases with endometriosis confirmed by laparoscopy and pathology and excluded the other infertile factors in Department of Reproductive Medicine in Peking University Shenzhen Hospital were enrolled in this study, which were divided into 17 cases with stage I-II, 19 cases with stage III-IV according to the revised classification American Fertility Society. Forty-four healthy women with male factor infertility were chosen as control group. The relative expression levels of mir-29c, mir-200a, mir-145 in the endometrium of women in two groups were detected by using real-time quantitative polymerase chain reaction. Those women were followed up for pregnancy outcome of endometriosis group after assisted reproductive techniques (pregnancy and non-pregnancy group respectively).
(1) The expression of miRNA between endometriosis and control groups:the average expression level of mir-29c, mir-200a, mir-145 in the endometrium of endometriosis group was 2.46 ± 1.98, 3.20 ± 2.45, 6.378 ± 3.275, which were significantly higher than 1.36 ± 1.05, 2.04 ± 1.16, 4.548 ± 1.885 in control group (P = 0.026, 0.027, 0.041, respectively). (2) The expression of miRNA between I-II stage and III-IV: the average expression level of mir-29c, mir-200a, mir-145 in the endometrium was 0.53 ± 0.51, 0.33 ± 0.26, 0.048 ± 0.021 in patients with of stage I-II, while 0.26 ± 0.18, 0.28 ± 0.12, 0.045 ± 0.016 in stage III-IV respectively, there were no statistically significant differences between the two groups (P = 0.191,0.661,0.753, respectively) . (3) The expression of miRNA between pregnancy and non-pregnancy groups:the average expression level of mir-29c, mir-200a, mir-145 in the endometrium were 0.60 ± 0.30, 1.23 ± 0.48, 0.886 ± 0.238 in pregnancy group, while 2.64 ± 1.73, 4.39 ± 2.58, 7.199 ± 3.945 in non pregnancy group, there were statistically significant differences between the two groups (P = 0.030,0.039,0.028, respectively).
Up-regulation of mir-29c, mir-200a, mir-145 in the endometrial tissue might play a role in endometriosis associated infertility.
研究微小RNA（miRNA）包括mir-29c、mir-200a、mir-145在分泌中期子宫内膜中的表达差异及其与子宫内膜异位症不孕的关系。
选取2011年8月至2013年2月在北京大学深圳医院生殖医学科经腹腔镜及病理确诊为子宫内膜异位症且排除其他不孕因素的36例不孕患者，根据美国生育协会修订分类法分为I-II期17例、III-IV期19例。选取44例因男方因素不孕的健康女性作为对照组。采用实时定量聚合酶链反应检测两组女性子宫内膜中mir-29c、mir-200a、mir-145的相对表达水平。对子宫内膜异位症组患者进行辅助生殖技术后的妊娠结局随访（分为妊娠组和未妊娠组）。
（1）子宫内膜异位症组与对照组miRNA表达情况：子宫内膜异位症组中mir-29c、mir-200a、mir-145的平均表达水平分别为2.46±1.98、3.20±2.45、6.378±3.275，显著高于对照组的1.36±1.05、2.04±1.16、4.548±1.885（P值分别为0.026、0.027、0.041）。（2）I-II期与III-IV期miRNA表达情况：I-II期患者子宫内膜中mir-29c、mir-200a、mir-145的平均表达水平分别为0.53±0.51、0.33±0.26、0.048±0.021，III-IV期分别为0.26±0.18、0.28±0.12、0.045±0.016，两组间差异无统计学意义（P值分别为0.191、0.661、0.753）。（3）妊娠组与未妊娠组miRNA表达情况：妊娠组子宫内膜中mir-29c、mir-200a、mir-145的平均表达水平分别为0.60±0.30、1.23±0.48、0.886±0.238，未妊娠组分别为
