[北京急性呼吸道感染儿童中人类鼻病毒不同种属感染情况]
[Different species of human rhinovirus infection in children with acute respiratory tract infections in Beijing].
作者信息
Song Ming-hui, Zhao Lin-qing, Qian Yuan, Zhu Ru-nan, Deng Jie, Wang Fang, Sun Yu, Tian Run
机构信息
Laboratory of Virology, Capital Institute of Pediatrics, Beijing 100020, China.
Laboratory of Virology, Capital Institute of Pediatrics, Beijing 100020, China. Email:
出版信息
Zhonghua Er Ke Za Zhi. 2013 Dec;51(12):903-8.
OBJECTIVE
To understand the clinical characteristics of different groups human rhinovirus (HRV)-A, B and C infection in children with acute respiratory tract infections (ARI) in Beijing.
METHOD
Respiratory tract specimens (n = 1412) collected from children with ARI during Jan. 2011 to Dec. 2012 were tested for HRV by using semi-nested PCR. Gene fragments of VP4/VP2 capsid protein amplified from HRV positive specimens were sequenced for HRV genotype confirmation. Then epidemiological characteristics of these HRV-positive cases were analyzed.
RESULT
Among these 1412 specimens tested, 103 (7.3%) were HRV positive, including 54 (52.4%) positive for HRV-A, 14 (13.6%) for HRV-B, 35 (34.0%) for HRV-C determined by sequence analysis. The positive rates of HRV-A, B and C (2.5%, 16/638; 0.3%, 2/638 and 1.3%, 8/638) in children with acute upper respiratory tract infections (URI) were lower than those (5.8%, 36/623; 1.8%, 11/623 and 3.9%, 24/623) in children with acute lower respiratory tract infections (LRI) (P = 0.003, 0.011, 0.003). In children with LRI, the positive rates of HRV-A, C were similar to each other (P = 0.112), and both were higher than that of HRV-B (P = 0.000, P = 0.026). The severity of ARI among children positive for different groups HRV showed no significant difference evaluated by Kruskal-Wallis H test (Hc = 0.044, P > 0.05), as well as that between children co-infected with HRV and other viruses and those infected with HRV only evaluated by Wilcoxon rank sum test (Zc = 0.872, P > 0.05).
CONCLUSION
HRV is one of important pathogens for children with ARI, especially LRI in Beijing. The positive rates of HRV-A and HRV-C are similar to each other, and both are higher than that of HRV-B. No significant difference was shown among children with different HRV genotypes by evaluation of the severity of ARI, and co-infections of HRV with other viruses do not significantly increase the severity of ARI.
目的
了解北京地区急性呼吸道感染（ARI）儿童中不同组别人鼻病毒（HRV）-A、B和C感染的临床特征。
方法
采用半巢式PCR对2011年1月至2012年12月期间收集的1412例ARI儿童呼吸道标本进行HRV检测。对HRV阳性标本扩增的VP4/VP2衣壳蛋白基因片段进行测序以确认HRV基因型。然后分析这些HRV阳性病例的流行病学特征。
结果
在检测的1412份标本中，103份（7.3%）HRV阳性，其中序列分析确定HRV-A阳性54份（52.4%），HRV-B阳性14份（13.6%），HRV-C阳性35份（34.0%）。急性上呼吸道感染（URI）儿童中HRV-A、B和C的阳性率（2.5%，16/638；0.3%，2/638和1.3%，8/638）低于急性下呼吸道感染（LRI）儿童（5.8%，36/623；1.8%，11/623和3.9%，24/623）（P = 0.003，0.011，0.003）。在LRI儿童中，HRV-A和C的阳性率彼此相似（P = 0.112），且均高于HRV-B（P = 0.000，P = 0.026）。通过Kruskal-Wallis H检验评估，不同组HRV阳性儿童中ARI的严重程度无显著差异（Hc = 0.044，P > 0.05），通过Wilcoxon秩和检验评估，HRV与其他病毒合并感染儿童和仅感染HRV儿童之间也无显著差异（Zc = 0.872，P > 0.05）。
结论
HRV是北京地区ARI儿童尤其是LRI儿童的重要病原体之一。HRV-A和HRV-C的阳性率彼此相似，且均高于HRV-B。评估ARI严重程度时，不同HRV基因型儿童之间无显著差异，HRV与其他病毒合并感染并未显著增加ARI的严重程度。
Zotero 插件