Zhang Shuang, Mao Nai-Ying, Yu De-Shan, Huang Guo-Hong, Li Xiao-Xia, Li Hong-Yu, Li Bao-Di, Zhang Yan, Cui Ai-Li, Chen Xiang-Peng, Yu Ai-Lian, Xu Wen-Bo
Pathogen Biology Teaching and Research Section, Taishan Medical University, Taian 271016, China.
Bing Du Xue Bao. 2013 May;29(3):273-9.
To study the epidemic characteristics of human rhinovirus (HRV) in children with acute respiratory infections in Gansu Province. 286 throat swabs were collected from children with acute respiratory in fections in Gansu Province during 2011. Multiplex reverse transcription-PCR (multiplex RT-PCR) assay was used to screen those specimens for detection of common respiratory tract pathogens. For HRV-positive samples, nested reverse transcription polymerase chain reaction (nested RT-PCR) was performed to amplify VP1 and VP4/VP2 gene fragments of HRV. The VP4/VP2 and VP1 regions of HRV-positive samples were sequenced and performed genotype analysis. Of 286 specimens fested, 27 were positive for HRV by multiplex RT-PCR and nested RT-PCR, of which 16 children were made (16/185), 8.64%) and 11 female (11/101,10.89%). The positive rate was 9.44% (27/286). The mean age of HRV-positive children was 3 years in this study, children less than one year old had the highest proportion 44.4% (12/ 27, 44.4%). The highest HRV positive rate fell on May, 2011 (6/27, 22.2%). Common cold accounted for the highest proportion, 12.24% (12/98) followed by pneumonia, 8.50% (13/153). The remaining 2 cases were bronchitis. Sequence analysis showed HRV A was the predominant genotype in Gansu Province in 2011, accounting for 84.62% (22/26) of positive cases, followed by HRV C (11.54%, 3/26) and only one HRV B was detected (3.85%, 1/26). HRV could be detected throughout the year in Gansu Province and primarily infected children under one year old. The group A was the epidemic genotype of HRV and move than one genotype existed in Gansu Province during 2011.
为研究甘肃省急性呼吸道感染儿童中人鼻病毒（HRV）的流行特征。2011年期间从甘肃省急性呼吸道感染儿童中采集了286份咽拭子。采用多重逆转录-聚合酶链反应（多重RT-PCR）检测方法对这些标本进行常见呼吸道病原体检测。对于HRV阳性样本，进行巢式逆转录聚合酶链反应（巢式RT-PCR）以扩增HRV的VP1和VP4/VP2基因片段。对HRV阳性样本的VP4/VP2和VP1区域进行测序并进行基因分型分析。在检测的286份标本中，27份经多重RT-PCR和巢式RT-PCR检测为HRV阳性，其中男童16例（16/185，8.64%），女童11例（11/101，10.89%）。阳性率为9.44%（27/286）。本研究中HRV阳性儿童的平均年龄为3岁，1岁以下儿童所占比例最高，为44.4%（12/27，44.4%）。HRV阳性率最高出现在2011年5月（6/27，22.2%）。普通感冒所占比例最高，为12.24%（12/98），其次是肺炎，为8.50%（13/153）。其余2例为支气管炎。序列分析显示HRV A是2011年甘肃省的主要基因型，占阳性病例的84.62%（22/26），其次是HRV C（11.54%，3/26），仅检测到1株HRV B（3.85%，1/26）。甘肃省全年均可检测到HRV，主要感染1岁以下儿童。A组是HRV的流行基因型，2011年甘肃省存在多种基因型。
