Ding Ya-Ming, Liu Zhuo, Li Fu-Qiang
The Second Hospital of Jilin University, Changchun, 130041, People's Republic of China.
Biomed Chromatogr. 2014 Sep;28(9):1205-11. doi: 10.1002/bmc.3147. Epub 2014 Feb 4.
A sensitive and accurate liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of dryocrassin ABBA, a potential active component isolated from Dryopteris crassirhizoma, in rat plasma. Chromatographic separation was achieved on a Zorbax SB-C18 column (50 × 2.1 mm, 1.8 µm), with elution consisting of eluent (A) 10 mm ammonium acetate in methanol containing 0.1% formic acid and (B) 10 mm ammonium acetate in water containing 0.1% formic acid (A:B = 99:1, v/v) at a flow rate of 0.3 mL/min. Multiple reaction monitoring mode was used to monitor the precursor-product ion transitions of m/z 819.3 → 403.4 for dryocrassin ABBA and m/z 426.2 → 409.2 for internal standard. This assay exhibited a good linearity with a correlation coefficient >0.99 and showed no endogenous interference with the analyte and internal standard. The lower limit of quantification of dryocrassin ABBA was 4 ng/mL in 50 μL of rat plasma. The method was successfully applied in the pharmacokinetic study of dryocrassin ABBA in rats after intravenous (2.35 mg/kg) and oral (23.5 mg/kg) doses of dryocrassin ABBA. The oral bioavailability (F) of dryocrassin ABBA was estimated to be 50.1%. Our study is the first to clarify the pharmacokinetic behaviors of dryocrassin ABBA in animals.
建立并验证了一种灵敏、准确的液相色谱 - 串联质谱法，用于测定大鼠血浆中粗茎鳞毛蕨中分离得到的潜在活性成分粗茎鳞毛蕨素ABBA。色谱分离在Zorbax SB - C18柱（50×2.1 mm，1.8 µm）上进行，洗脱液由（A）含0.1%甲酸的甲醇中10 mM醋酸铵和（B）含0.1%甲酸的水中10 mM醋酸铵组成（A:B = 99:1，v/v），流速为0.3 mL/min。采用多反应监测模式监测粗茎鳞毛蕨素ABBA的m/z 819.3→403.4和内标的m/z 426.2→409.2的前体 - 产物离子跃迁。该方法具有良好的线性，相关系数>0.99，且对分析物和内标无内源性干扰。粗茎鳞毛蕨素ABBA在50 μL大鼠血浆中的定量下限为4 ng/mL。该方法成功应用于粗茎鳞毛蕨素ABBA在大鼠静脉注射（2.35 mg/kg）和口服（23.5 mg/kg）剂量后的药代动力学研究。粗茎鳞毛蕨素ABBA的口服生物利用度（F）估计为50.1%。我们的研究首次阐明了粗茎鳞毛蕨素ABBA在动物体内的药代动力学行为。
