Tome Yasunori, Uehara Fuminari, Mii Sumiyuki, Yano Shuya, Zhang Lei, Sugimoto Naotoshi, Maehara Hiroki, Bouvet Michael, Tsuchiya Hiroyuki, Kanaya Fuminori, Hoffman Robert M
AntiCancer, Inc., 7917 Ostrow Street, San Diego, California, 92111; Department of Surgery, University of California San Diego, 200 West Arbor Drive, San Diego, California, 92103; Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara Nishihara, Okinawa, 903-0125, Japan.
J Cell Biochem. 2014 Aug;115(8):1362-7. doi: 10.1002/jcb.24780.
Cell and tissue culture can be performed on different substrates such as on plastic, in Matrigel™, and on Gelfoam(®), a sponge matrix. Each of these substrates consists of a very different surface, ranging from hard and inflexible, a gel, and a sponge-matrix, respectively. Folkman and Moscona found that cell shape was tightly coupled to DNA synthesis and cell growth. Therefore, the flexibility of a substrate is important for cells to maintain their optimal shape. Human osteosarcoma cells, stably expressing a fusion protein of α(v) integrin and green fluorescent protein (GFP), grew as a simple monolayer without any structure formation on the surface of a plastic dish. When the osteosarcoma cells were cultured within Matrigel™, the cancer cells formed colonies but no other structures. When the cancer cells were seeded on Gelfoam(®), the cells formed three-dimensional tissue-like structures. The behavior of 143B osteosarcoma cells on Gelfoam(®) in culture is remarkably different from those of these cells in monolayer culture or in Matrigel™. Tissue-like structures were observed only in Gelfoam(®) culture. The data in this report suggest a flexible structural substrate such as Gelfoam(®) provides a more in vivo-like culture condition than monolayer culture or Matrigel(TM) and that Matrigel(TM) does not result in actual three-dimensional culture.
细胞和组织培养可以在不同的基质上进行，如塑料、基质胶（Matrigel™）和明胶海绵（Gelfoam(®)）（一种海绵基质）。这些基质中的每一种都具有非常不同的表面，分别从坚硬且不可弯曲的表面、凝胶和海绵基质不等。福尔克曼和莫斯科纳发现细胞形状与DNA合成和细胞生长紧密相关。因此，基质的柔韧性对于细胞维持其最佳形状很重要。稳定表达α(v)整合素与绿色荧光蛋白（GFP）融合蛋白的人骨肉瘤细胞，在塑料培养皿表面呈简单单层生长，没有形成任何结构。当骨肉瘤细胞在基质胶（Matrigel™）中培养时，癌细胞形成集落但没有其他结构。当癌细胞接种在明胶海绵（Gelfoam(®)）上时，细胞形成三维组织样结构。143B骨肉瘤细胞在培养中的明胶海绵（Gelfoam(®)）上的行为与这些细胞在单层培养或基质胶（Matrigel™）中的行为显著不同。仅在明胶海绵（Gelfoam(®)）培养中观察到组织样结构。本报告中的数据表明，像明胶海绵（Gelfoam(®)）这样的柔性结构基质比单层培养或基质胶（Matrigel(TM)）提供了更类似体内的培养条件，并且基质胶（Matrigel(TM)）不会导致实际的三维培养。
