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抗草甘膦刺苋（刺苋）中5-烯醇丙酮酰莽草酸-3-磷酸合成酶（EPSPS）基因扩增：一例通过种间杂交从抗草甘膦糙果苋（糙果苋）进行基因转移的案例

EPSPS amplification in glyphosate-resistant spiny amaranth (Amaranthus spinosus): a case of gene transfer via interspecific hybridization from glyphosate-resistant Palmer amaranth (Amaranthus palmeri).

作者信息

Nandula Vijay K, Wright Alice A, Bond Jason A, Ray Jeffery D, Eubank Thomas W, Molin William T

机构信息

Crop Production Systems Research Unit, USDA-ARS, Stoneville, MS, USA.

出版信息

Pest Manag Sci. 2014 Dec;70(12):1902-9. doi: 10.1002/ps.3754. Epub 2014 Mar 20.

DOI:10.1002/ps.3754
PMID:24497375
Abstract

BACKGROUND

Amaranthus spinosus, a common weed of pastures, is a close relative of Amaranthus palmeri, a problematic agricultural weed with widespread glyphosate resistance. These two species have been known to hybridize, allowing for transfer of glyphosate resistance. Glyphosate-resistant A. spinosus was recently suspected in a cotton field in Mississippi.

RESULTS

Glyphosate-resistant A. spinosus biotypes exhibited a fivefold increase in resistance compared with a glyphosate-susceptible biotype. EPSPS was amplified 33-37 times and expressed 37 times more in glyphosate-resistant A. spinosus biotypes than in a susceptible biotype. The EPSPS sequence in resistant A. spinosus plants was identical to the EPSPS in glyphosate-resistant A. palmeri, but differed at 29 nucleotides from the EPSPS in susceptible A. spinosus plants. PCR analysis revealed similarities between the glyphosate-resistant A. palmeri amplicon and glyphosate-resistant A. spinosus.

CONCLUSIONS

Glyphosate resistance in A. spinosus is caused by amplification of the EPSPS gene. Evidence suggests that part of the EPSPS amplicon from resistant A. palmeri is present in glyphosate-resistant A. spinosus. This is likely due to a hybridization event between A. spinosus and glyphosate-resistant A. palmeri somewhere in the lineage of the glyphosate-resistant A. spinosus plants. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.

摘要

背景

刺苋是牧场常见杂草，是具广泛草甘膦抗性的有害农业杂草——帕尔默苋的近缘种。已知这两个物种会杂交，从而使草甘膦抗性得以转移。最近在密西西比州的一块棉田中怀疑出现了抗草甘膦的刺苋。

结果

与草甘膦敏感型生物型相比，抗草甘膦的刺苋生物型抗性提高了五倍。抗草甘膦的刺苋生物型中5-烯醇丙酮酰莽草酸-3-磷酸合成酶（EPSPS）扩增了33 - 37倍，表达量是敏感型生物型的37倍。抗草甘膦的刺苋植株中的EPSPS序列与抗草甘膦的帕尔默苋中的EPSPS相同，但与敏感型刺苋植株中的EPSPS在29个核苷酸处存在差异。聚合酶链反应（PCR）分析显示抗草甘膦的帕尔默苋扩增子与抗草甘膦的刺苋之间存在相似性。

结论

刺苋中的草甘膦抗性是由EPSPS基因扩增引起的。有证据表明，抗草甘膦的刺苋中存在部分来自抗草甘膦的帕尔默苋的EPSPS扩增子。这可能是由于在抗草甘膦的刺苋植株谱系中的某个位置，刺苋与抗草甘膦的帕尔默苋发生了杂交事件。2014年发表。本文是美国政府作品，在美国属于公共领域。

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