Kaushik P, Kaushik D, Yadav J, Pahwa P
Institute of Phramaceutical sciences, Kurukshetra University, Kurukshetra, India.
Pak J Biol Sci. 2013 Aug 15;16(16):804-11. doi: 10.3923/pjbs.2013.804.811.
The activity of the alcoholic extract of the rhizomes of Alpinia galanga was studied for the treatment of diabetes-induced nephropathy in rats. Wistar rats received a single intraperitoneal streptozotocin injection (60 mg kg(-1) b.wt.) to induce diabetes. Rats were considered diabetic if blood glucose concentration increased up to 200 or more mg dL(-1). The rats were orally administered alcoholic extract of Alpinia galanga (50, 100 and 200 mg kg(-1)), once daily for 40 days. Body weight, blood glucose, urinary albumin, glycosylated haemoglobin, Blood Urea Nitrogen (BUN), creatinine, lipids profile, Malondialdehyde (MDA), Superoxide Dismutase (SOD), Glutathione (GSH) and Catalase (CAT) were then evaluated. After 40 days of treatment, Alpinia galanga significantly (p<0.05) decreased glycaemia, Blood Urea Nitrogen (BUN), urinar albumin and increased body weight in diabetes-nephropathic rats. The extract (200 mg kg(-1)) decreased MDA significantly (p<0.01); GSH (p<0.05), increased SOD (p<0.05) and CAT (p<0.05) in the rats, compared with nephropathic control. The extract (100 and 200 mg kg(-1), respectively) lowered (p<0.05) total cholesterolemia, blood triglycerides (p<0.05), blood LDL cholesterol (p<0.05), but increased blood HDL cholesterol (p<0.01). Overall, atherogenic index was decreased significantly (p<0.05). In the present study, the rhizomes of Alpinia galanga demonstrated significant nephro-protective activities in the tested models. The alcoholic extract of the rhizomes of Alpinia galanga holds promise for the development of a standardized phytomedicine for diabetes mellitus and kidney disease treatment.
研究了高良姜根茎乙醇提取物对大鼠糖尿病肾病的治疗作用。将Wistar大鼠腹腔注射一次链脲佐菌素(60 mg kg(-1)体重)以诱导糖尿病。若血糖浓度升高至200 mg dL(-1)或更高,则认为大鼠患有糖尿病。大鼠每天口服高良姜乙醇提取物(50、100和200 mg kg(-1)),持续40天。然后评估体重、血糖、尿白蛋白、糖化血红蛋白、血尿素氮(BUN)、肌酐、血脂谱、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)和过氧化氢酶(CAT)。治疗40天后,高良姜显著(p<0.05)降低了糖尿病肾病大鼠的血糖、血尿素氮(BUN)、尿白蛋白,并增加了体重。与肾病对照组相比,提取物(200 mg kg(-1))显著(p<0.01)降低了MDA;增加了大鼠的GSH(p<0.05)、SOD(p<0.05)和CAT(p<0.05)。提取物(分别为100和200 mg kg(-1))降低了(p<0.05)总胆固醇血症、血甘油三酯(p<0.05)、血低密度脂蛋白胆固醇(p<0.05),但增加了血高密度脂蛋白胆固醇(p<0.01)。总体而言,动脉粥样硬化指数显著降低(p<0.05)。在本研究中,高良姜根茎在测试模型中表现出显著的肾保护活性。高良姜根茎乙醇提取物有望开发成为一种标准化的植物药,用于治疗糖尿病和肾脏疾病。