Doğan Abdulahad, Çelik İsmail
a Faculty of Science, Department of Biology , Yuzuncu Yil University , Van , Turkey ;
b Faculty of Science, Department of Molecular Biology and Genetics , Yuzuncu Yil University , Van , Turkey.
Pharm Biol. 2016 Oct;54(10):2092-102. doi: 10.3109/13880209.2016.1145702. Epub 2016 Mar 9.
Context Sumac [Rhus coriaria L. (RC) (Anacardiaceae)] is used as a folk medicine in the treatment of diabetes in Turkey. Objective This study investigates the in vivo healing and protective effects of lyophilized extract sumac against streptozotocin (STZ)-induced diabetic complications. Materials and methods Toxicity test was conducted in three different dosages (250, 500 and 1000 mg/kg of plant extracts, respectively). Six groups of seven rats each were used in experiments. Groups were designed as Normal control, Diabetic (DM), DM + AC-20 mg/kg, DM + Extract-100 mg/kg, DM + Extract 250 mg/kg and DM + Extract 500 mg/kg group. Experimental diabetes [50 mg/kg, intraperitoneal (i.p.)] was induced by STZ. The effects of oral administration of the extract for 21 d on the level of serum glucose, insulin, C-peptide, lipid profile (LP), hepatic and renal damage biomarkers (HRDB), diabetic serum biomarkers (DSB), glycosylated haemoglobin (HbA1c), antioxidant defence system constituents (ADSCs), malondialdehyde (MDA) and α-glucosidase activity in small intestine tissue were evaluated. Results The extract decreased the levels of blood glucose in diabetic groups (an average of 31%). Triglyceride, total cholesterol, high-density lipoprotein and low-density lipoprotein levels were balanced by plant extract (500 mg/kg) supplementation in the diabetic group. Decreased levels of aspartate aminotransferase (89%), alanine aminotransferase (91%), lactate dehydrogenase (35%), alkaline phosphatase (47%), creatinine (25%) and urea (29%) were detected in plant extract (500 mg/kg) supplemented diabetic group. Additionally, a considerable increase in the HRDB, DSB, LP, MDA and fluctuated ADSC levels were restored in RC-extract supplemented groups. Conclusion RC lyophilized extract has a healing effect on diabetes and diabetes-related complications.
背景 漆树[盐肤木（RC）（漆树科）]在土耳其被用作治疗糖尿病的民间药物。目的 本研究调查了冻干漆树提取物对链脲佐菌素（STZ）诱导的糖尿病并发症的体内愈合和保护作用。材料与方法 以三种不同剂量（分别为250、500和1000mg/kg植物提取物）进行毒性试验。实验中使用了六组，每组七只大鼠。分组设计为正常对照组、糖尿病组（DM）、糖尿病+AC-20mg/kg组、糖尿病+提取物-100mg/kg组、糖尿病+提取物250mg/kg组和糖尿病+提取物500mg/kg组。通过STZ诱导实验性糖尿病[50mg/kg，腹腔注射（i.p.）]。评估连续21天口服提取物对血清葡萄糖、胰岛素、C肽、血脂谱（LP）、肝和肾损伤生物标志物（HRDB）、糖尿病血清生物标志物（DSB）、糖化血红蛋白（HbA1c）、抗氧化防御系统成分（ADSCs）、丙二醛（MDA）和小肠组织中α-葡萄糖苷酶活性的影响。结果 提取物降低了糖尿病组的血糖水平（平均降低31%）。糖尿病组补充植物提取物（500mg/kg）可使甘油三酯、总胆固醇、高密度脂蛋白和低密度脂蛋白水平恢复平衡。在补充植物提取物（500mg/kg）的糖尿病组中，检测到天冬氨酸转氨酶（降低89%）、丙氨酸转氨酶（降低91%）、乳酸脱氢酶（降低35%）、碱性磷酸酶（降低47%）、肌酐（降低25%）和尿素（降低29%）的水平下降。此外，补充RC提取物的组中，HRDB、DSB、LP、MDA和波动的ADSC水平显著升高的情况得到了恢复。结论 RC冻干提取物对糖尿病及糖尿病相关并发症具有愈合作用。
