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肾移植中CD14+CD16+和CD14+CD163+单核细胞亚群

CD14+CD16+ and CD14+CD163+ monocyte subpopulations in kidney allograft transplantation.

作者信息

Sekerkova Alena, Krepsova Eva, Brabcova Eva, Slatinska Janka, Viklicky Ondrej, Lanska Vera, Striz Ilja

机构信息

Department of Clinical and Transplant Immunology, Institute for Clinical and Experimental Medicine, Videnska 1958/9, Prague 4 140 21, Czech Republic.

出版信息

BMC Immunol. 2014 Feb 6;15:4. doi: 10.1186/1471-2172-15-4.

DOI:10.1186/1471-2172-15-4
PMID:24499053
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3918100/
Abstract

BACKGROUND

Monocytes represent a heterogeneous population of cells subdivided according to the expression level of membrane antigens. A pro-inflammatory (intermediate/nonclassical) subpopulation of monocytes is defined by expression of CD16. CD163 seems to be characteristically preferentially expressed by immunosuppressive monocytes. The aim of our study was to evaluate the distribution of monocyte subpopulations in 71 patients with kidney allograft transplantation.

RESULTS

The phenotype was evaluated by flow cytometry in defined time points. The proportions of peripheral CD14+CD16+ monocytes were downregulated immediately after the kidney transplantation and basiliximab treatment partially attenuated this trend. The transient downregulation of the CD14+CD16+ subpopulation was adjusted to basal values in two months. The proportions of CD14+CD163+ monocytes were transiently upregulated early after the kidney transplantation and remained higher during the first month in most patients. In ATG treated patients, the expansion of CD14+CD163+ monocytes was delayed but their upregulation lasted longer. In vitro data showed the direct effect of ATG and methylprednisolone on expression of CD16 and CD163 molecules while basiliximab did not affect the phenotype of cultured monocytes.

CONCLUSIONS

We assume from our data that kidney allograft transplantation is associated with modulation of monocyte subpopulations (CD14+CD16+ and CD14+CD163+) partially affected by an immunosuppressive regime used.

摘要

背景

单核细胞是一类异质性细胞群体，可根据膜抗原表达水平进行细分。促炎性（中间型/非经典型）单核细胞亚群由CD16的表达来定义。CD163似乎是免疫抑制性单核细胞的特征性优先表达分子。我们研究的目的是评估71例肾移植患者单核细胞亚群的分布情况。

结果

在特定时间点通过流式细胞术评估细胞表型。肾移植后外周血CD14+CD16+单核细胞比例立即下调，巴利昔单抗治疗部分减弱了这一趋势。CD14+CD16+亚群的短暂下调在两个月内恢复至基础值。肾移植后早期CD14+CD163+单核细胞比例短暂上调，且在大多数患者的第一个月内持续较高。在接受抗胸腺细胞球蛋白（ATG）治疗的患者中，CD14+CD163+单核细胞的扩增延迟，但其上调持续时间更长。体外数据显示ATG和甲泼尼龙对CD16和CD163分子的表达有直接影响，而巴利昔单抗不影响培养单核细胞的表型。

结论

根据我们的数据，我们推测肾移植与单核细胞亚群（CD14+CD16+和CD14+CD163+）的调节有关，这种调节部分受所用免疫抑制方案的影响。

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