a Department of Veterinary Clinical and Animal Sciences , Faculty of Health and Medical Sciences, University of Copenhagen , Frederiksberg C , Denmark.
Arch Anim Nutr. 2014;68(1):42-54. doi: 10.1080/1745039X.2014.880554.
To get more knowledge about the energy requirements of dogs and to formulate appropriate feeding guidelines, it is essential to determine their energy expenditure (EE) in a reliable and feasible way. In this study, the non-invasive oral stable isotope (13)C-bicarbonate technique (o(13)CBT) was validated against indirect calorimetry (IC) for the determination of CO2-production and EE in dogs. Eleven privately owned dogs were simultaneously measured with IC and the o(13)CBT after being fasted overnight. All dogs were measured twice on two separate days. For calculation, measurements were divided into two groups depending on dogs' behaviour during the measurement. Dogs of Group 1 (n = 17) were resting calmly in the chamber and dogs of Group 2 (n = 5) were more active. Mean heart rate was significantly higher in Group 2 (102 beats per minute [bpm]) than in Group 1 (77 bpm) (p < 0.001). Within groups, the CO2-production and EE [kJ d(-1) kg BW(-0.75)] estimated by the o(13)CBT or IC did not differ significantly (Group 1: [Formula: see text] = 368; EEIC = 363; Group 2: [Formula: see text] = 701; EEIC = 718). However, the estimated (13)C recovery factor (RF) for the estimation of CO2-production was significantly different between Groups 1 and 2 (0.72 and 0.94, respectively, p < 0.001). The respiratory quotient (RQ), which is needed for the estimation of EE, did not differ between groups. This study shows that the non-invasive o(13)CBT can be used for accurate estimation of the CO2-production rate and EE in resting dogs. A value of 0.77 can be applied as an estimate of the RQ in fasted dogs and 0.72 as an appropriate estimate for RF when dogs are resting calmly during the measurements.
为了更深入地了解犬类的能量需求，并制定相应的喂养指南，我们必须以可靠且可行的方式确定其能量消耗（EE）。在这项研究中，我们对非侵入性口腔稳定同位素（13）C-碳酸氢盐技术（o(13)CBT）进行了验证，以间接测热法（IC）来测定犬类的 CO2 产生和 EE。11 只私人拥有的犬只在禁食过夜后，同时接受 IC 和 o(13)CBT 测量。所有犬只在两个不同的日子进行了两次测量。为了计算，根据犬只在测量过程中的行为，将测量结果分为两组。第 1 组（n=17）的犬只在腔室内安静地休息，第 2 组（n=5）的犬只更活跃。第 2 组的平均心率明显高于第 1 组（102 次/分钟[bpm]比 77 bpm）（p<0.001）。在每组内，o(13)CBT 或 IC 估计的 CO2 产生量和 EE[kJ d(-1) kg BW(-0.75)]没有显著差异（第 1 组：[公式：见文本] = 368；EEIC = 363；第 2 组：[公式：见文本] = 701；EEIC = 718）。然而，估计 CO2 产生的（13）C 回收率（RF）在第 1 组和第 2 组之间存在显著差异（分别为 0.72 和 0.94，p<0.001）。用于估计 EE 的呼吸商（RQ）在组间没有差异。本研究表明，非侵入性的 o(13)CBT 可用于准确估计静息犬类的 CO2 产生率和 EE。在禁食犬中，可采用 0.77 作为 RQ 的估计值，在犬类在测量过程中安静休息时，可采用 0.72 作为 RF 的适当估计值。
