Watanabe M, Watts T H, Gariepy J, Hozumi N
Mount Sinai Hospital Research Institute, Toronto, Ontario, Canada.
Cell Immunol. 1988 Mar;112(1):226-35. doi: 10.1016/0008-8749(88)90291-2.
The expression and function of surface immunoglobulin (sIg) receptors was analyzed using antigen-specific monoclonal B cells and T cells. When B cells were incubated with antigen (hapten carrier), hapten-specific sIg receptor expression was greatly reduced. The specific antigen-presentation activity of the B cells was also markedly reduced. Following the removal of antigen, expression of the sIg receptors was restored to original levels within 3-4 hr. Specific antigen-presentation activity of these B cells was concomitantly restored. Reduction of sIg receptor expression and specific antigen presentation were inhibited by the addition of competing free hapten, suggesting that crosslinking of sIg receptors is required for the observed changes in sIg receptor expression and that specific antigen presentation is hapten specific. Using oligopeptide-specific Th cells, we could show the requirement for processing of antigen in the interaction between hapten-specific B cells and carrier-specific T cells. These results indicated that the expression of sIg receptors for a specific antigen and the ability to present the antigen are interrelated.
利用抗原特异性单克隆B细胞和T细胞分析了表面免疫球蛋白（sIg）受体的表达及功能。当B细胞与抗原（半抗原载体）一起孵育时，半抗原特异性sIg受体的表达显著降低。B细胞的特异性抗原呈递活性也明显降低。去除抗原后，sIg受体的表达在3 - 4小时内恢复到原始水平。这些B细胞的特异性抗原呈递活性也随之恢复。添加竞争性游离半抗原可抑制sIg受体表达的降低和特异性抗原呈递，这表明sIg受体的交联是观察到的sIg受体表达变化所必需的，并且特异性抗原呈递具有半抗原特异性。使用寡肽特异性Th细胞，我们可以证明在半抗原特异性B细胞与载体特异性T细胞之间的相互作用中对抗原加工的需求。这些结果表明，针对特定抗原的sIg受体的表达与呈递该抗原的能力是相互关联的。
