Degwert J, Heuer J, Kölsch E
Department of Immunology, University of Münster, Federal Republic of Germany.
J Immunol. 1988 Mar 1;140(5):1448-53.
Two BSA-specific Ts cell clones have been isolated from CBA/J mice tolerized by low doses of BSA. Together with one Ts cell clone isolated from an immune animal, they have recently been characterized with regard to phenotypes and in vitro functions. In the present report the in vivo effector functions of two of them (Ts cell clones BVI/5 and HF1.MS) are described. BSA-primed lymph node cells from CBA/J mice, which had received BVI/5 or HF1.MS Ts cells at the time of immunization, do not respond to a subsequent in vitro antigenic challenge. A human gamma-globulin (HGG)-specific lymph node cell proliferation is not influenced. BVI/5 Ts cells injected into mice at the time of priming with fluorescein (Flu)-conjugated BSA or Flu-HGG inhibit the humoral anti-Flu-response in Flu-BSA-primed animals. The anti-Flu-response in Flu-HGG-primed animals is only marginally affected by BVI/5 Ts cells. The data show that it is possible to induce immunologic unresponsiveness at the humoral level by reexposing in vitro propagated Ts cell clones to their syngenic in vivo environment.
从经低剂量牛血清白蛋白（BSA）耐受的CBA/J小鼠中分离出了两个BSA特异性T抑制细胞（Ts细胞）克隆。连同从免疫动物中分离出的一个Ts细胞克隆一起，它们最近在表型和体外功能方面得到了鉴定。在本报告中，描述了其中两个克隆（Ts细胞克隆BVI/5和HF1.MS）的体内效应功能。在免疫时接受了BVI/5或HF1.MS Ts细胞的CBA/J小鼠的BSA致敏淋巴结细胞，对随后的体外抗原刺激无反应。人γ球蛋白（HGG）特异性淋巴结细胞增殖不受影响。在用荧光素（Flu）偶联的BSA或Flu-HGG进行初次免疫时将BVI/5 Ts细胞注射到小鼠体内，可抑制Flu-BSA致敏动物的体液抗Flu反应。BVI/5 Ts细胞对Flu-HGG致敏动物的抗Flu反应仅有轻微影响。数据表明，通过将体外增殖的Ts细胞克隆重新暴露于其同基因的体内环境中，有可能在体液水平诱导免疫无反应性。
