Stuart P M, Zlotnik A, Woodward J G
Department of Microbiology and Immunology, Albert B. Chandler Medical Center, Lexington, KY 40536.
J Immunol. 1988 Mar 1;140(5):1542-7.
We have studied the effects of IL-4 (B cell stimulatory factor 1) on the expression of MHC gene products in normal bone marrow-derived macrophages, peritoneal macrophages, and the myelomonocytic cell line WEHI-3. Using both IL-4-containing T cell supernatant and rIL-4, we have observed significant induction of both class I and class II MHC surface expression (about 1.5- to 4-fold increase) in 2-, 3-, and 4-day cultures of bone marrow-derived macrophages. This induction was also apparent at the mRNA level as assessed by Northern blot analysis using A beta, E alpha, and class I probes. Kinetic analysis revealed that induction of class II mRNA by IL-4 was slower than induction by IFN-gamma, requiring 48 h before a significant increase was noted. The magnitude of MHC induction by IL-4 was not as great as that seen with IFN-gamma, which was found to increase surface expression of MHC antigens two- to eightfold. IL-4 also differs from IFN-gamma in the repertoire of macrophages responsive to it. IL-4 was unable to induce class I or class II expression in either thioglycolate-elicited peritoneal macrophages or WEHI-3 cells whereas IFN-gamma induced MHC antigen expression on both cell types under the same conditions. These data demonstrate that IL-4 is capable of inducing both class I and class II MHC gene products in some, but not all, macrophages.
我们研究了白细胞介素-4（B细胞刺激因子1）对正常骨髓来源的巨噬细胞、腹腔巨噬细胞以及骨髓单核细胞系WEHI-3中MHC基因产物表达的影响。使用含白细胞介素-4的T细胞上清液和重组白细胞介素-4，我们观察到在骨髓来源巨噬细胞的2天、3天和4天培养物中，I类和II类MHC表面表达均有显著诱导（增加约1.5至4倍）。通过使用Aβ、Eα和I类探针的Northern印迹分析评估，这种诱导在mRNA水平也很明显。动力学分析显示，白细胞介素-4诱导II类mRNA的速度比干扰素-γ慢，在显著增加之前需要48小时。白细胞介素-4诱导MHC的幅度不如干扰素-γ大，后者可使MHC抗原的表面表达增加2至8倍。白细胞介素-4在对其有反应的巨噬细胞种类方面也与干扰素-γ不同。白细胞介素-4无法在巯基乙酸盐诱导的腹腔巨噬细胞或WEHI-3细胞中诱导I类或II类表达，而干扰素-γ在相同条件下可在这两种细胞类型上诱导MHC抗原表达。这些数据表明，白细胞介素-4能够在部分而非全部巨噬细胞中诱导I类和II类MHC基因产物。
