Tammi R, Ripellino J A, Margolis R U, Tammi M
Department of Anatomy, University of Kuopio, Finland.
J Invest Dermatol. 1988 Mar;90(3):412-4. doi: 10.1111/1523-1747.ep12456530.
Hyaluronate is actively synthesized by cultured epidermis and dermis, but no direct histological data have been available about its localization in normal human skin. A hyaluronate-specific biotinylated probe, prepared from the hyaluronate binding region of cartilage proteoglycan, was applied to human skin sections and visualized using the biotin-avidin-peroxidase system. The specificity of this staining was confirmed by hyaluronidase predigestion and by hyaluronate-derived oligosaccharides added to the staining solution. All dermis showed diffuse binding of the probe, but the highest staining intensity was observed in the epidermal intercellular spaces. The stainability extended from basal cells to the middle layers of the epidermis, whereas the granular layer and stratum corneum were completely negative. Also, the basal side of basal cells (basement membrane) did not bind the hyaluronate probe. The abundance of hyaluronate on surfaces and intercellular spaces of the spinous cells is suggested to have an important role in the physiology of human epidermis.
透明质酸盐可由培养的表皮和真皮积极合成，但尚无关于其在正常人体皮肤中定位的直接组织学数据。一种由软骨蛋白聚糖的透明质酸盐结合区域制备的透明质酸盐特异性生物素化探针，应用于人体皮肤切片，并使用生物素-抗生物素蛋白-过氧化物酶系统进行可视化观察。这种染色的特异性通过透明质酸酶预消化以及添加到染色溶液中的透明质酸盐衍生寡糖得以证实。所有真皮均显示出探针的弥漫性结合，但在表皮细胞间隙中观察到最高的染色强度。染色性从基底细胞延伸至表皮中层，而颗粒层和角质层则完全呈阴性。此外，基底细胞的基底侧（基底膜）不结合透明质酸盐探针。棘细胞表面和细胞间隙中丰富的透明质酸盐被认为在人体表皮生理中具有重要作用。
