Goldberg R L
CIBA-GEIGY Corporation, Research Department, Summit, New Jersey 07901.
Anal Biochem. 1988 Nov 1;174(2):448-58. doi: 10.1016/0003-2697(88)90043-7.
An enzyme-linked immunosorbent assay has been developed to measure hyaluronate concentrations in biological samples. The assay is based on the aggregation of hyaluronate with cartilage proteoglycan monomer, followed by binding of a monoclonal antibody to the keratan sulfate on the proteoglycan. The sensitivity of the assay is 10 ng hyaluronate/ml of either serum or conditioned cell culture medium. The coefficient of variability was between 10 and 20%. Hyaluronate added to samples was recovered quantitatively and digestion of cell culture medium with protease did not affect the concentration of hyaluronate. Hyaluronate polysaccharides as small as a decasaccharide can be measured. This sensitive and convenient assay can be used for measuring large numbers of biological samples from a variety of animal and tissue sources.
已开发出一种酶联免疫吸附测定法来测量生物样品中的透明质酸盐浓度。该测定法基于透明质酸盐与软骨蛋白聚糖单体的聚集，随后单克隆抗体与蛋白聚糖上的硫酸角质素结合。该测定法的灵敏度为10 ng透明质酸盐/血清或条件性细胞培养基每毫升。变异系数在10%至20%之间。添加到样品中的透明质酸盐可被定量回收，用蛋白酶消化细胞培养基不会影响透明质酸盐的浓度。小至十糖的透明质酸多糖都可被测量。这种灵敏且便捷的测定法可用于测量来自各种动物和组织来源的大量生物样品。
