Askelöf P, Rodmalm K, Abens J, Undén A, Bartfai T
Department of Production, National Bacteriological Laboratory, Stockholm, Sweden.
J Infect Dis. 1988 Apr;157(4):738-42. doi: 10.1093/infdis/157.4.738.
We synthesized six peptides (15-22 amino acids) from the amino acid sequence of the S1 subunit of pertussis toxin. The antigenicity of the polypeptides was investigated by enzyme-linked immunosorbent assays, in which the polypeptides coupled to bovine serum albumin were used as coating antigens. The polypeptides were examined as antigens for reaction with pre- and postvaccination sera from infants receiving either a Bordetella pertussis whole-cell vaccine or a vaccine containing pertussis toxoid. An elevated serum titer was noted upon vaccination with both types of vaccines when bovine serum albumin conjugates of five synthetic peptides were used as antigens. Similarly, mice immunized with whole-cell pertussis vaccine showed an elevated titer against all five peptides that were reactive with human sera. Thus, we identified five peptide sequences of S1 against which, in two species, antibodies are formed upon exposure of these species to whole cells of B. pertussis.
我们从百日咳毒素S1亚基的氨基酸序列合成了六种肽（15 - 22个氨基酸）。通过酶联免疫吸附测定法研究了这些多肽的抗原性，其中与牛血清白蛋白偶联的多肽用作包被抗原。将这些多肽作为抗原，检测其与接种百日咳全细胞疫苗或含百日咳类毒素疫苗的婴儿接种前和接种后的血清的反应。当使用五种合成肽的牛血清白蛋白缀合物作为抗原时，接种两种疫苗后均观察到血清滴度升高。同样，用全细胞百日咳疫苗免疫的小鼠对与人类血清反应的所有五种肽的滴度也升高。因此，我们鉴定出了S1的五个肽序列，在两个物种中，当这些物种暴露于百日咳博德特氏菌全细胞时会针对它们形成抗体。
