Wiethölter H, Hülser P J, Linington C, Meier D H, Wessel K
Neurologische Klinik Universität Tübingen, FRG.
J Neurol Sci. 1988 Jan;83(1):1-14. doi: 10.1016/0022-510x(88)90015-9.
Acute experimental allergic neuritis (EAN) was produced in Lewis rats by transfer of lymphocytes from a permanent T cell line specific for bovine P2 protein. In 3 groups of rats receiving 10(4), 10(5) and 10(6) total injected P2-specific lymphocytes, respectively, the time course of illness was followed by measuring several electrophysiological parameters including the H reflex or F wave and lumbospinal somatosensory evoked potentials (SEP). The severity and time course of both the electrophysiological and clinical (e.g., loss of weight and development of paresis) parameters of illness depended on the number of injected lymphocytes. Lower numbers of injected cells were correlated with a later onset and less severe symptoms as well as with an earlier and more complete recovery. According to clinical observation EAN mediated by lymphocytes is a monophasic illness. According to our electrophysiological measurements, however, the disease can be described by the following successive stages: (a) an early stage of hyperexcitability; (b) a stage of acute partial conduction block; (c) 14 days later a stage of maximal demyelination; and (d) a recovery phase. Although demyelination is the prominent feature of the disease, axonal degeneration also occurs to an extent directly related to the number of cells injected. Degeneration was not observed in rats from the group with the lowest number (10(4] of injected lymphocytes.
通过转移对牛P2蛋白特异的永久性T细胞系的淋巴细胞，在Lewis大鼠中诱发急性实验性变应性神经炎（EAN）。在分别接受10⁴、10⁵和10⁶个总注射量的P2特异性淋巴细胞的3组大鼠中，通过测量包括H反射或F波以及腰脊髓体感诱发电位（SEP）在内的几个电生理参数来跟踪疾病的时间进程。疾病的电生理和临床（如体重减轻和轻瘫的发展）参数的严重程度和时间进程取决于注射淋巴细胞的数量。注射细胞数量较少与发病较晚、症状较轻以及恢复较早且较完全相关。根据临床观察，淋巴细胞介导的EAN是一种单相疾病。然而，根据我们的电生理测量，该疾病可描述为以下连续阶段：（a）早期的兴奋性增高阶段；（b）急性部分传导阻滞阶段；（c）14天后的最大脱髓鞘阶段；以及（d）恢复阶段。虽然脱髓鞘是该疾病的突出特征，但轴索变性也在一定程度上发生，且与注射细胞的数量直接相关。在注射淋巴细胞数量最低（10⁴）的组的大鼠中未观察到变性。
