Bozkurt Mehtap, Em Serda, Oktayoglu Pelin, Turkcu Gul, Yuksel Hatice, Sarıyıldız Mustafa A, Caglayan Mehmet, Batmaz Ibrahim, Nas Kemal, Bozkurt Yasar, Kuyumcu Mahir
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Clin Invest Med. 2014 Feb 1;37(1):E19-25. doi: 10.25011/cim.v37i1.20865.
The purpose of this study was to investigate the effect of carvacrol (CAR) on methotrexate (MTX)-induced renal damage in rats.
Twenty-four male rats were equally divided into three groups: group I, control treatment; group II, MTX-treated; and group III, MTX+CAR-treated. A single dose of CAR (73 mg/kg) was administered intraperitoneally to group III on the first day of the experiment and a single dose of MTX (20 mg/kg) was administered intraperitoneally to groups II and III on the second day of the experiment. Blood samples and kidney tissue were obtained from each animal on day 8 for the measurement of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI). Light microscopy was used for histopathological examination of kidney specimens.
MDA, TOS and OSI levels were significantly greater in the group receiving MTX alone relative to the control animals, while the TAS level was significantly reduced in the MTX group compared with the control group. The administration of CAR was associated with significantly decreased MDA, TOS, and OSI levels and increased TAS levels relative to the rats treated with MTX alone. Animals treated with CAR exhibited decreased tubular degeneration and architectural impairment relative to animals treated with MTX alone; however, the difference in histological scores did not meet the threshold of statistical significance.
MTX treatment results in oxidative damage to the rat kidney; damage which is partially abrogated by the administration of CAR.
本研究旨在探讨香芹酚（CAR）对甲氨蝶呤（MTX）诱导的大鼠肾损伤的影响。
将24只雄性大鼠平均分为三组：第一组为对照处理组；第二组为MTX处理组；第三组为MTX+CAR处理组。在实验第一天，对第三组腹腔注射单剂量的CAR（73mg/kg），在实验第二天，对第二组和第三组腹腔注射单剂量的MTX（20mg/kg）。在第8天从每只动物采集血样和肾组织，用于测定丙二醛（MDA）、总氧化剂状态（TOS）、总抗氧化剂状态（TAS）和氧化应激指数（OSI）。用光镜对肾脏标本进行组织病理学检查。
单独接受MTX的组中，MDA、TOS和OSI水平相对于对照动物显著更高，而MTX组的TAS水平与对照组相比显著降低。与单独用MTX治疗的大鼠相比，给予CAR与MDA、TOS和OSI水平显著降低以及TAS水平升高有关。与单独用MTX治疗的动物相比，用CAR治疗的动物肾小管变性和结构损伤减少；然而，组织学评分的差异未达到统计学意义阈值。
MTX治疗导致大鼠肾脏氧化损伤；给予CAR可部分消除这种损伤。
