Nouri Ali, Izak-Shirian Farzad, Fanaei Vahideh, Dastan Maryam, Abolfathi Mahdieh, Moradi Alireza, Khaledi Mansoor, Mirshekari-Jahangiri Hamzeh
Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Medical Plant Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Heliyon. 2021 Nov 11;7(11):e08358. doi: 10.1016/j.heliyon.2021.e08358. eCollection 2021 Nov.
Diclofenac (DIC) is an NSAID that can cause toxic effects in animals and humans and carvacrol (CAR) is a monoterpene compound that displays effective pharmacological and biological actions. The purpose of this work was to assess the influences of CAR on DIC-induced renal injury and oxidative stress in male rats. The rats were segregated into four groups. Group 1, control group; Group 2 received DIC-only; Groups 3, received CAR-only and group 4 received DIC plus CAR. Changes in biochemical indexes, pathological changes, molecular biological indexes, and genes related to the inflammation of main organs were evaluated. The results of this work indicated that the amounts of the serum protein carbonyl, sGOT, sGPT, urea, creatinine, uric acid, nitrite content, MDA, serum TNF-α, and renal TNF-α gene expression were remarkably increased and the levels of the GPx, GSH, CAT, and SOD were significantly reduced in DIC-only treated animals compared to the control group. On the other hand, treatment with CAR after exposure to DIC led to significant improvements in abnormalities of DIC-induced renal injury and serum biochemical factors. The data approve that CAR diminished the deleterious effects of DIC exposure. In this regard, the findings of this study indicated that the administration of CAR could alleviate the noxious effects of DIC on the antioxidant defense system and renal tissue.
双氯芬酸（DIC）是一种非甾体抗炎药，可对动物和人类产生毒性作用，而香芹酚（CAR）是一种单萜化合物，具有有效的药理和生物学作用。本研究的目的是评估CAR对DIC诱导的雄性大鼠肾损伤和氧化应激的影响。将大鼠分为四组。第1组为对照组；第2组仅接受DIC；第3组仅接受CAR，第4组接受DIC加CAR。评估了主要器官的生化指标、病理变化、分子生物学指标以及与炎症相关的基因变化。本研究结果表明，与对照组相比，仅接受DIC治疗的动物血清蛋白羰基、sGOT、sGPT、尿素、肌酐、尿酸、亚硝酸盐含量、丙二醛、血清肿瘤坏死因子-α（TNF-α）以及肾TNF-α基因表达量显著增加，而谷胱甘肽过氧化物酶（GPx）、谷胱甘肽（GSH）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）水平显著降低。另一方面，在暴露于DIC后用CAR治疗可显著改善DIC诱导的肾损伤和血清生化因子异常。数据证实CAR减轻了DIC暴露的有害影响。在这方面，本研究结果表明，给予CAR可减轻DIC对抗氧化防御系统和肾组织的有害影响。
