Toran-Allerand C D
Center for Reproductive Sciences, Columbia University College of Physicians and Surgeons, New York, NY 10032.
Neurosci Lett. 1987 Dec 16;83(1-2):35-40. doi: 10.1016/0304-3940(87)90212-6.
Homologous explants of embryonic day (E)-17 fetal liver and brain (hypothalamus and preoptic area) were co-cultured in juxtaposition for up to 2 weeks in vitro. Hepatocytes were shown to contain immunoreactive alpha-fetoprotein (AFP) by immunofluorescence, as were neurons differentiating in the outgrowth zones in contact with the liver explants. No immunoreactive intraneuronal AFP was observed in liver cultures grown alone or in CNS cultures grown in the absence of liver or exogenously added AFP. This more physiological paradigm provides strong confirming evidence for the extracellular origin of the intraneuronal AFP observed during development and extends earlier observations on the uptake of purified mouse AFP added in vivo and in vitro.
将胚胎第17天（E-17）的胎肝与脑（下丘脑和视前区）的同源外植体并列共培养长达2周。通过免疫荧光显示，肝细胞含有免疫反应性甲胎蛋白（AFP），与肝外植体接触的生长区域中分化的神经元也是如此。在单独培养的肝培养物中，或在没有肝脏或未添加外源性AFP的情况下培养的中枢神经系统培养物中，未观察到免疫反应性神经元内AFP。这种更接近生理状态的模式为发育过程中观察到的神经元内AFP的细胞外起源提供了有力的确证证据，并扩展了之前关于体内和体外添加的纯化小鼠AFP摄取的观察结果。
