Sklarin P M, Awdeh Z L, Alper C A
Center for Blood Research, Boston, Mass.
Vox Sang. 1988;54(1):43-6. doi: 10.1111/j.1423-0410.1988.tb01611.x.
An immunoblotting technique allows direct visualization of Chido and Rodgers antigenic determinants on intact C4 proteins. C4 molecules separated by electrophoresis are selectively transferred to nitrocellulose membranes saturated with goat antiserum to human C4. The membranes are then incubated in alloanti-Chido or anti-Rodgers followed by enzyme-conjugated goat antihuman IgG. Molecules with Chido or Rodgers reactivity are visualized by incubation with an indicator substrate for the bound enzyme.
免疫印迹技术可直接观察完整C4蛋白上的奇多和罗杰斯抗原决定簇。通过电泳分离的C4分子被选择性转移到用山羊抗人C4血清饱和的硝酸纤维素膜上。然后将膜在同种抗奇多或抗罗杰斯中孵育，接着用酶联山羊抗人IgG孵育。具有奇多或罗杰斯反应性的分子通过与结合酶的指示底物孵育来观察。
