Borras-Cuesta F, Fedon Y, Petit-Camurdan A
Institut National de la Recherche Agronomique, Station de Recherches de Virologie et d'Immunologie, Thiverval-Grignon, France.
Eur J Immunol. 1988 Feb;18(2):199-202. doi: 10.1002/eji.1830180203.
The effect of linear homopolymerization on the immunogenicity of synthetic peptides was studied using either haptenic peptides (representing amino acid sequences 103-115 and 133-147 of bovine rotavirus major protein) or immunogenic peptides TD-103-115 and TD-133-147 which were constructed by co-linear synthesis of the former peptides and an amino acid sequence representing a determinant recognized by T helper cells (TD). It was found that the two haptenic peptides were rendered immunogenic by linear homopolymerization. Moreover, homopolymerization also enhanced the immunogenicity of TD-103-115 but not that of TD-133-147. In the three cases where polymerization enhanced immunogenicity, a reinforced amphipathic pattern was predicted in the neighborhood of the junction of the monomers. The possibility that polymerization might have generated a new T cell determinant is discussed.
利用半抗原肽（代表牛轮状病毒主要蛋白的氨基酸序列103 - 115和133 - 147）或免疫原性肽TD - 103 - 115和TD - 133 - 147研究了线性均聚对合成肽免疫原性的影响，后者是通过前一种肽与代表辅助性T细胞（TD）识别的决定簇的氨基酸序列共线合成构建的。发现这两种半抗原肽通过线性均聚而具有免疫原性。此外，均聚还增强了TD - 103 - 115的免疫原性，但未增强TD - 133 - 147的免疫原性。在聚合增强免疫原性的三种情况下，预测在单体连接处附近会出现增强的两亲性模式。讨论了聚合可能产生新的T细胞决定簇的可能性。
