含大肠杆菌 LT 的免疫刺激贴剂增强了针对 DNA 和重组蛋白阿尔茨海默病疫苗的免疫反应。
Immunostimulant patches containing Escherichia coli LT enhance immune responses to DNA- and recombinant protein-based Alzheimer's disease vaccines.
机构信息
Department of Molecular Immunology, Institute for Molecular Medicine, Huntington Beach, CA 92647, USA.
Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, CA 92697, USA.
出版信息
J Neuroimmunol. 2014 Mar 15;268(1-2):50-7. doi: 10.1016/j.jneuroim.2014.01.002. Epub 2014 Jan 15.
Abstract
Immunotherapeutic approaches to treating Alzheimer's disease (AD) using vaccination strategies must overcome the obstacle of achieving adequate responses to vaccination in the elderly. Here we demonstrate for the first time that application of the Escherichia coli heat-labile enterotoxin adjuvant-laden immunostimulatory patches (LT-IS) dramatically enhances the onset and magnitude of immune responses to DNA- and protein-based vaccines for Alzheimer's disease following intradermal immunization via gene gun and conventional needles, respectively. Our studies suggest that the immune activation mediated by LT-IS offers improved potency for generating AD-specific vaccination responses that should be investigated as an adjuvant in the clinical arena.
摘要
免疫疗法治疗阿尔茨海默病（AD）的方法，利用疫苗接种策略，必须克服在老年人中实现疫苗接种充分反应的障碍。在这里，我们首次证明，在经皮基因枪和常规针免疫接种后，应用大肠杆菌不耐热肠毒素佐剂负载的免疫刺激贴剂（LT-IS）可显著增强针对阿尔茨海默病的 DNA 和蛋白质疫苗的免疫反应的出现和强度。我们的研究表明，LT-IS 介导的免疫激活提供了增强 AD 特异性疫苗接种反应的效力，应作为佐剂在临床领域进行研究。
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