电穿孔与基因枪传递阿尔茨海默病 DNA 疫苗产生强大且相似的免疫反应。
Delivery of a DNA vaccine for Alzheimer's disease by electroporation versus gene gun generates potent and similar immune responses.
机构信息
Department of Molecular Immunology, Institute for Molecular Medicine, Huntington Beach, Calif 92647, USA.
出版信息
Neurodegener Dis. 2012;10(1-4):261-4. doi: 10.1159/000333359. Epub 2012 Feb 1.
Abstract
BACKGROUND
Induction of a humoral response against amyloid-β peptide may be beneficial for Alzheimer's disease (AD) patients and may alleviate the onset and progression of AD. DNA-based vaccination provides a unique alternative method of immunization for treatment and prevention of AD. Currently, the two major delivery methods used for enhancing DNA uptake and immune responses to DNA vaccines in humans are electroporation (EP) and gene gun (GG).
OBJECTIVE
The goal of this translational study was to evaluate the efficacy of an AD DNA epitope vaccine (DepVac) delivered intramuscularly by EP or intradermally by GG.
METHODS
Humoral and cellular immune responses to immunization with DepVac were evaluated by ELISA and ELISPOT, respectively. Functional activity of the antibodies was also assessed.
RESULTS
EP- and GG-mediated immunizations with DepVac induced similar anti-amyloid-β (Aβ) antibody and T cell responses. Anti-Aβ antibodies bound to amyloid plaques in AD brain tissue and to toxic forms of Aβ(42) peptide.
CONCLUSION
Both delivery methods are effective at promoting potent antibodies specific for Aβ.
摘要
背景
诱导针对淀粉样蛋白-β肽的体液反应可能对阿尔茨海默病（AD）患者有益，并可能减轻 AD 的发病和进展。基于 DNA 的疫苗接种为 AD 的治疗和预防提供了一种独特的免疫接种替代方法。目前，用于增强人类对 DNA 疫苗的 DNA 摄取和免疫反应的两种主要递送方法是电穿孔（EP）和基因枪（GG）。
目的
本转化研究的目的是评估 AD DNA 表位疫苗（DepVac）通过 EP 肌肉内给药或 GG 皮内给药的疗效。
方法
通过 ELISA 和 ELISPOT 分别评估免疫接种 DepVac 后的体液和细胞免疫反应。还评估了抗体的功能活性。
结果
EP 和 GG 介导的 DepVac 免疫诱导了相似的抗淀粉样蛋白-β（Aβ）抗体和 T 细胞反应。抗 Aβ 抗体与 AD 脑组织中的淀粉样斑块以及有毒形式的 Aβ（42）肽结合。
结论
两种递送方法都能有效地促进针对 Aβ 的强效抗体。
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