丹酚酸B对大鼠心肌缺血再灌注损伤的作用及机制

Effect and mechanism of salvianolic acid B on the myocardial ischemia-reperfusion injury in rats.

作者信息

Xue Ling, Wu Zhen, Ji Xiao-Ping, Gao Xia-Qing, Guo Yan-Hua

机构信息

Department of Cardiovascular Medicine, Qilu Hospital, Shandong University, No 107 Wenhua Xi Road, Jinan 250012, P.R. China; Department of Cardiovascular Medicine, Third Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, Liaoning Province, China.

Department of Cardiovascular Medicine, Third Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, Liaoning Province, China.

出版信息

Asian Pac J Trop Med. 2014 Apr;7(4):280-4. doi: 10.1016/S1995-7645(14)60038-9.

DOI:10.1016/S1995-7645(14)60038-9

PMID:24507676

Abstract

OBJECTIVE

To investigate the effect of salvianolic acid B on rats with myocardial ischemia-reperfusion injury.

METHODS

SD rats were randomly divided into five groups (n=10 in each group): A sham operation group, B ischemic reperfusion group model group, C low dose salvianolic acid B group, D median dose salvianolic acid B group, E high dose salvianolic acid B group. One hour after establishment of the myocardial ischemia-reperfusion model, the concentration and the apoptotic index of the plasma level of myocardial enzymes (CTn I, CK-MB), SOD, MDA, NO, ET were measured. Heart tissues were obtained and micro-structural changes were observed.

RESULTS

Compared the model group, the plasma CTn, CK-MB, MDA and ET contents were significantly increased, NO, T-SOD contents were decreased in the treatment group (group C, D, and E) (P<0.05); compared with group E, the plasma CTn I, CK-MB, MDA and ET levels were increased, the NO, T-SOD levels were decreased in groups C and D (P<0.05). Infarct size was significantly reduced, and the myocardial ultrastructural changes were improved significantly in treatment group.

CONCLUSIONS

Salvianolic acid B has a significant protective effect on myocardial ischemia-reperfusion injury. It can alleviate oxidative stress, reduce calcium overload, improve endothelial function and so on.

摘要

目的

探讨丹酚酸B对心肌缺血再灌注损伤大鼠的影响。

方法

将SD大鼠随机分为五组（每组n = 10）：A假手术组、B缺血再灌注模型组、C丹酚酸B低剂量组、D丹酚酸B中剂量组、E丹酚酸B高剂量组。建立心肌缺血再灌注模型1小时后，测定心肌酶（CTn I、CK-MB）、超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）、内皮素（ET）血浆水平的浓度及凋亡指数。获取心脏组织并观察微观结构变化。

结果

与模型组相比，治疗组（C、D、E组）血浆CTn、CK-MB、MDA及ET含量显著升高，NO、总超氧化物歧化酶（T-SOD）含量降低（P<0.05）；与E组相比，C组和D组血浆CTn I、CK-MB、MDA及ET水平升高，NO、T-SOD水平降低（P<0.05）。治疗组梗死面积显著减小，心肌超微结构变化明显改善。