Yang Fu-guo, Zhang An-yong, Chen Zuo-yuan, Lian Zhe-xun, Liu Ge-xin, Dong Guo-xiong
Department of Internal Medicine, Nursing School, Qingdao University Medical College, Qingdao, Shandong Province 266021, China.
Zhong Xi Yi Jie He Xue Bao. 2008 Dec;6(12):1250-4. doi: 10.3736/jcim20081208.
To investigate the effects of salvianolic acid B (SA-B) on cardiovascular endothelial cell function and platelet activation during myocardial ischemia-reperfusion in rabbits.
A total of 24 New Zealand white rabbits were randomly divided into sham-operated group, ischemia-reperfusion group (untreated group) and SA-B group. The hearts of rabbits in untreated group and SA-B group underwent half an hour of left anterior descending coronary artery (LADCA) occlusion via ligation technology, which was followed by 4 hours of reperfusion to prepared ischemia-reperfusion injury model in vivo. For sham-operated group, the animals were not subjected to occlusion of LADCA. In SA-B treatment group the rabbits were intravenously administered SA-B immediately after LADCA occlusion, and the other two groups were given normal saline in the same way instead of SA-B. The jugular vein bloods of animals were collected before LADCA ligation, half an hour after ligation and after 1-, 4-hour reperfusion, respectively. The content of plasma nitric oxide (NO) was determined by nitrate reductase process. Radioimmunoassay was applied to detect the endothelin (ET) content in plasma and the count of alpha-granule membrane protein-140 (GMP-140) on platelet surface to identify the activation of the platelet.
No significant difference was observed before and after sham LADCA occlusion in sham-operated group in the contents of NO and ET in plasma (P>0.05), neither was the count of GMP-140 on platelet surface (P>0.05). The content of NO in plasma detected 0.5 h after LADCA occlusion was significantly decreased in untreated group compared with the sham-operated group at the corresponding time, and they were also much lower than that before LADCA occlusion in the sham-operated group (P<0.05). The plasma content of NO in untreated group showed a progressive decrease in response to the myocardial reperfusion. However, the content of ET in plasma and the count of GMP-140 on platelet surface were remarkably increased after myocardial ischemia as compared with those before LADCA ligation and those detected in sham-operated group (P<0.05). The content of ET and the count of GMP-140 in the untreated group were further increased corresponding to the aggressive reperfusion. The content of NO was significantly increased while the content of ET and the count of GMP-140 were both significantly decreased in SA-B group as compared with untreated group after 1- and 4-hour myocardial reperfusion, respectively (P<0.01).
The results show that endothelial dysfunction and platelet activation occur during ischemia-reperfusion in rabbit hearts in vivo and SA-B protects cardiovascular endothelium cells against ischemia-reperfusion injury and inhibits the activation of platelet during myocardial ischemia and reperfusion.
探讨丹酚酸B（SA-B）对兔心肌缺血再灌注过程中心血管内皮细胞功能及血小板活化的影响。
将24只新西兰白兔随机分为假手术组、缺血再灌注组（未治疗组）和SA-B组。未治疗组和SA-B组兔心脏通过结扎技术进行半小时的左冠状动脉前降支（LADCA）闭塞，随后进行4小时再灌注，以制备体内缺血再灌注损伤模型。假手术组动物不进行LADCA闭塞。SA-B治疗组兔在LADCA闭塞后立即静脉注射SA-B，另外两组以同样方式给予生理盐水而非SA-B。分别在LADCA结扎前、结扎后半小时及再灌注1小时、4小时后采集动物颈静脉血。采用硝酸还原酶法测定血浆一氧化氮（NO）含量。应用放射免疫分析法检测血浆内皮素（ET）含量及血小板表面α-颗粒膜蛋白-140（GMP-140）计数，以鉴定血小板活化情况。
假手术组LADCA闭塞前后血浆中NO和ET含量无显著差异（P>0.05），血小板表面GMP-140计数也无显著差异（P>0.05）。与假手术组相应时间相比，未治疗组LADCA闭塞0.5小时后检测的血浆NO含量显著降低，且也远低于假手术组LADCA闭塞前水平（P<0.05）。未治疗组血浆NO含量随心肌再灌注呈进行性下降。然而，与LADCA结扎前及假手术组检测结果相比，心肌缺血后血浆ET含量及血小板表面GMP-140计数显著增加（P<0.05）。未治疗组ET含量及GMP-140计数随再灌注加重进一步增加。与未治疗组相比，SA-B组在心肌再灌注1小时和4小时后，NO含量显著增加，而ET含量及GMP-140计数均显著降低（P<0.01）。
结果表明，兔心脏体内缺血再灌注过程中发生内皮功能障碍和血小板活化，SA-B可保护心血管内皮细胞免受缺血再灌注损伤，并抑制心肌缺血和再灌注期间血小板的活化。
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