视网膜干细胞联合COP-1对青光眼大鼠模型视神经损伤的影响
Effect of RSCs combined with COP-1 on optic nerve damage in glaucoma rat model.
作者信息
Fu Wei-Cai, Jiang Yan, Zhang Lin
机构信息
Department of Ophthalmology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
Department of Ophthalmology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
出版信息
Asian Pac J Trop Med. 2014 Apr;7(4):317-20. doi: 10.1016/S1995-7645(14)60047-X.
OBJECTIVE
To explore effect of retinal stem cells (RSCs) combined with copolymer-1 (COP-1) immunotherapy on optic nerve damage in glaucoma rat model.
METHODS
A total of 40 SD rats were selected for glaucoma model and were randomly divided into 4 groups to observe protective effects of RSCs transplantation combined with COP-1.
RESULTS
Brain-derived neurotrophic factor (BDNF) and insulin like growth factor-1 (IGF-1) were either positive in retina of RSCs transplanted or COP-1 immunological treated rat. Positive rate of BDNF and IGF-1 and expression of mRNA and protein were significantly higher in RSCs transplantation combined with COP-1 immunotherapy treated rats compared with the other 3 groups, in which amount of apoptotic RGCs was lowest.
CONCLUSIONS
RSCs transplantation combined with COP-1 immunotherapy can promote the secretion of BDNF and IGF-1. They protect RGCs in glaucoma rats in coordination, significantly reduce the number of apoptosis RGCs so as to alleviate the optic nerve damage. It ponits a new research direction for treatment of glaucoma.
目的
探讨视网膜干细胞（RSCs）联合共聚体-1（COP-1）免疫疗法对青光眼大鼠模型视神经损伤的影响。
方法
选取40只SD大鼠建立青光眼模型，随机分为4组，观察RSCs移植联合COP-1的保护作用。
结果
脑源性神经营养因子（BDNF）和胰岛素样生长因子-1（IGF-1）在RSCs移植或COP-1免疫治疗大鼠的视网膜中均呈阳性。与其他3组相比，RSCs移植联合COP-1免疫治疗大鼠的BDNF和IGF-1阳性率以及mRNA和蛋白表达显著更高，其中凋亡视网膜神经节细胞（RGCs）数量最低。
结论
RSCs移植联合COP-
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